Studies on the Antigenicity of Glucagon

• Published in: The Tohoku Journal of Experimental Medicine Vol. 141; no. 4; pp. 407 - 416
• Main Authors: KASAMA, TOSHIO, AIDA, YOKO, OSHIRO, KIICHIRO, GOTO, MACHIKO, UCHIDA, MICHIKO, OHKUNI, HISASHI
• Format: Journal Article
• Language: English
• Published: Japan Tohoku University Medical Press 01.01.1983
• Subjects: anaphylactic shock; Anaphylaxis - immunology; Animals; Antibodies - analysis; antiglucagon antibody; Arthus Reaction - immunology; Female; glucagon; Glucagon - adverse effects; Glucagon - immunology; Guinea Pigs; Immunoglobulin E - analysis; Male; Mice; Mice, Inbred BALB C; Passive Cutaneous Anaphylaxis; Radioimmunoassay; Rats; Rats, Inbred Strains; reaginic antibody; Reagins - analysis
• ISSN: 0040-8727; 1349-3329
• DOI: 10.1620/tjem.141.407

KASAMA, T., AIDA, Y., OSHIRO, K., GOTO, M., UCHIDA, M. and OHKUNI, H. Studies on the Antigenicity of Glucagon. Tohoku J. exp. Med., 1983, 141 (4), 407-415 - In order to test the safety of glucagon (GG), the immunogenicity of GG was studied in rabbits and guinea pigs. Any sensitization, as revealed by anaphylactic shock, Arthus reaction, passive cutaneous anaphylaxis (PCA) or radioimmunoassay, was not demonstrated in animals immunized with GG alone. The anti-GG antibodies were detected by PCA and radioimmunoassay in two of the five animals immunized with GG mixed with Freund's complete adjuvant. Anti-GG IgE antibody production in mice was found in one of the ten mice given 570μg and threeof the six mice given 1, 000μg of GG with alminum hydroxide gel (Alum), but other doses of GG with Alum produced no IgE antibody. These results indicate that the antigenicity of GG is very weak.