Sulforaphane decreases viability and telomerase activity in hepatocellular carcinoma Hep3B cells through the reactive oxygen species-dependent pathway

• Published in: Cancer letters Vol. 295; no. 2; pp. 260 - 266
• Main Authors: Moon, Dong-Oh, Kang, Sang-Hyuck, Kim, Ki-Cheon, Kim, Mun-Ock, Choi, Yung Hyun, Kim, Gi-Young
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 28.09.2010; Elsevier Limited
• Subjects: Aging; Anticarcinogenic Agents - pharmacology; Apoptosis; Apoptosis - drug effects; Biotechnology; Carcinoma, Hepatocellular - drug therapy; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Cell Line, Tumor; Cell Survival - drug effects; Down-Regulation; Hematology, Oncology and Palliative Medicine; Humans; Isothiocyanates; Liver cancer; Liver Neoplasms - drug therapy; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Oxygen; Phosphorylation; Proteins; Proto-Oncogene Proteins c-akt - metabolism; Reactive oxygen species; Reactive Oxygen Species - metabolism; Rodents; Sulforaphane; Telomerase; Telomerase - antagonists & inhibitors; Telomerase reverse transcriptase; Thiocyanates - pharmacology; Reactive oxygen species; Telomerase reverse transcriptase; Telomerase; Sulforaphane
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/j.canlet.2010.03.009

Abstract Sulforaphane (SFN), a dietary isothiocyanate, is a well known natural product that possesses anti-cancer and chemopreventive activities. However, the molecular mechanism of the anti-telomerase activity of SFN is not well understood. In this study, we investigated the hypothesis that SFN inhibits cell viability and telomerase activity via downregulation of telomerase reverse transcriptase (hTERT) expression. We suggest that elevated intracellular reactive oxygen species (ROS) levels, due to exposure to SFN, has a critical role in abolishing since pretreatment with NAC, an antioxidant, resulted in the recovery of hTERT expression. SFN also suppressed the phosphorylation of Akt (Ser-473), thereby inhibiting hTERT phosphorylation and this effect was reversed by pretreatment with NAC. Taken together, these data suggest that ROS are essential for the suppression of SFN-mediated telomerase activity via transcriptional and posttranslational regulation of hTERT.