Combined Black Phosphorus Nanosheets with ICG/aPDT is an Effective Anti-Inflammatory Treatment for Periodontal Disorders

• Published in: International journal of nanomedicine Vol. 18; pp. 813 - 827
• Main Authors: Li, Xincong, Ren, Shuangshuang, Song, Lutong, Gu, Deao, Peng, Haoran, Zhao, Yue, Liu, Chao, Yang, Jie, Miao, Leiying
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2023; Taylor & Francis Ltd; Dove; Dove Medical Press
• Subjects: Analysis; Animals; Anti-Bacterial Agents - pharmacology; Anti-inflammatory drugs; Antibacterial agents; antibacterial photodynamic therapy; Bacteria; black phosphorus nanosheets; Cancer; Cytokines; Disease; Drug resistance; Gum disease; Infections; Inflammation; Interleukin-10; Interleukin-6; Lasers; macrophage polarization; Nanomaterials; Original Research; Periodontal Diseases - drug therapy; periodontitis; Periodontitis - microbiology; Phosphorus; Photochemotherapy; Photochemotherapy - methods; Photosensitizing Agents - pharmacology; Rats; Reactive Oxygen Species; RNA; RNA, Messenger; Spectrum analysis; Transforming Growth Factor beta; Transforming growth factors; Tumor Necrosis Factor-alpha; Tumor necrosis factor-TNF; China; United States > US; Japan; macrophage polarization; antibacterial photodynamic therapy; black phosphorus nanosheets; periodontitis; reactive oxygen species
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S394861

Antibacterial photodynamic treatment (aPDT) has indispensable significance as a means of treating periodontal disorders because of its extraordinary potential for killing pathogenic bacteria by generating an overpowering amount of reactive oxygen species (ROS). The elevated ROS that may result from the antibacterial treatment procedure, however, could exert oxidative pressure inside periodontal pockets, causing irreparable damage to surrounding tissue, an issue that has severely restricted its medicinal applications. Accordingly, herein, we report the use of black phosphorus nanosheets (BPNSs) that can eliminate the side effects of ROS-based aPDT as well as scavenge ROS to produce an antibacterial effect. The antibacterial effect of ICG/aPDT was observed by direct microscopic colony counting. A microplate reader and confocal microscope enabled measurements of cell viability and the quantification of ROS fluorescence. BPNS administration regulated the oxidative environment. IL-1β, IL-6, TNF-α, IL-10, TGF-β, and Arg-1 mRNA expression levels were used to assess the inflammatory response after BPNS treatment. In vivo, the efficacy of the combination of BPNSs and ICG/aPDT was evaluated in rats with periodontal disease by histomorphometric and immunohistochemical analyses. The CFU assay results verified the antibacterial effect of ICG/aPDT treatment, and ROS fluorescence quantification by CLSM indicated the antioxidative ability of the BPNSs. IL-1β, IL-6, TNF-α, IL-10, TGF-β, and Arg-1 mRNA expression levels were significantly decreased after BPNS treatment, confirming the in vitro anti-inflammatory effect of this nanomaterial. The histomorphometric and immunohistochemical analyses showed that the levels of proinflammatory factors decreased, suggesting that the BPNSs had anti-inflammatory effects in vivo. Treatment with antioxidative BPNSs gives new insights into future anti-inflammatory therapies for periodontal disease and other infection-related inflammatory illnesses and provides an approach to combat the flaws of aPDT.