Proteomic changes in articular cartilage of human endemic osteoarthritis in China

• Published in: Proteomics (Weinheim) Vol. 11; no. 14; pp. 2881 - 2890
• Main Authors: Ma, Wei-Juan, Guo, Xiong, Liu, Jiang-Tao, Liu, Rui-Yu, Hu, Jian-Wen, Sun, An-Guo, Yu, Yue-Xiang, Lammi, Mikko J.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.07.2011; WILEY‐VCH Verlag; Wiley-VCH; Wiley Subscription Services, Inc
• Subjects: 2-D DIGE; Adult; Analytical, structural and metabolic biochemistry; Biochemistry; biokemi; Biological and medical sciences; Biomedicine; Cartilage; Cartilage, Articular - chemistry; Cartilage, Articular - cytology; Cartilage, Articular - pathology; cell research; cellforskning; Cells, Cultured; China; Chondrocytes - chemistry; Chondrocytes - cytology; Chondrocytes - metabolism; Computational Biology; Cytoskeleton; Databases, Protein; Diseases of the osteoarticular system; Female; Fundamental and applied biological sciences. Psychology; Humans; Kashin-Beck disease; Kashin-Beck Disease - metabolism; Kashin-Beck Disease - pathology; Male; Medical sciences; Middle Aged; Miscellaneous; Miscellaneous. Osteoarticular involvement in other diseases; Orthopaedics; ortopedi; Osteoarthritis; Osteoarthritis - metabolism; Osteoarthritis - pathology; Pathogenesis; Proteins; Proteins - analysis; Proteome - analysis; Proteomics - methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Two-Dimensional Difference Gel Electrophoresis - methods; China; Human; Cartilage; Biomedicine; Articular cartilage; 2-D DIGE; Diseases of the osteoarticular system; Kashin-Beck disease; Proteomics; Arthropathy; Environmental factor
• ISSN: 1615-9853; 1615-9861; 1862-8346; 1615-9861
• DOI: 10.1002/pmic.201000636

Kashin–Beck disease (KBD) is a chronic endemic osteochondropathy with unclear pathogenesis. It is a degenerative disease similar to osteoarthritis, but with different manifestations of cartilage damage. The aim of this investigation was to show the protein changes in KBD cartilage and to identify the candidate proteins in order to understand the pathogenesis of the disease. Proteins were extracted from the media of primary cell cultures of KBD and normal chondrocytes, and separated by two‐dimensional fluorescence difference gel electrophoresis (2‐D DIGE). MALDI‐TOF/TOF analysis revealed statistically significant differences in 27 proteins from KBD chondrocyte cultures, which consisted of 17 up‐regulated and ten down‐regulated proteins. The results were further validated by Western blot analysis. The proteins identified are mainly involved in cellular redox homeostasis and stress response (MnSOD, Hsp27, Peroxiredoxin‐1, and Cofilin‐1), glycolysis (PGK‐1, PGM‐1, α‐enolase), and cell motility and cytoskeletal organization (Actin, Calponin‐2, and Keratin). These KBD‐associated proteins indicate that cytoskeletal remodeling, glycometabolism, and oxidative stress are abnormal in KBD articular cartilage.