Salinity inhibits seed germination and embryo growth by reducing starch mobilization efficiency in barley
Barley is one of the world's earliest domesticated crops, which is widely used for beer production, animal feeding, and health care. Barley seed germination, particularly in increasingly saline soils, is key to ensure the safety of crop production. However, the mechanism of salt‐affected seed g...
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Published in | Plant direct Vol. 8; no. 2; pp. e564 - n/a |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
John Wiley & Sons, Inc
01.02.2024
Wiley |
Subjects | |
Online Access | Get full text |
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Summary: | Barley is one of the world's earliest domesticated crops, which is widely used for beer production, animal feeding, and health care. Barley seed germination, particularly in increasingly saline soils, is key to ensure the safety of crop production. However, the mechanism of salt‐affected seed germination in barley remains elusive. Here, two different colored barley varieties were used to independently study the regulation mechanism of salt tolerance during barley seed germination. High salinity delays barley seed germination by slowing down starch mobilization efficiency in seeds. The starch plate test revealed that salinity had a significant inhibitory effect on α‐amylase activity in barley seeds. Further, NaCl treatment down‐regulated the expression of Amy1, Amy2 and Amy3 genes in germinated seeds, thereby inhibiting α‐amylase activity. In addition, the result of embryogenic culture system in vitro showed that the shoot elongation of barley was significantly inhibited by salt stress. These findings indicate that it is a feasible idea to study the regulation mechanism of salinity on barley seed germination and embryo growth from the aspect of starch‐related source‐sink communication. |
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Bibliography: | Funding information This work was supported by funding from Collaborative Technology Popularization Plan for Yield and Quality of Malting Barley (2022‐ZYXT‐04‐2), Basic Science (Natural Science) Research Project of Higher Education Institutions of Jiangsu Province (23KJB210013), Industrial System Malting Barley Innovation Team Project of Jiangsu Modern Agriculture (Special Food and Special Economy) [JATS(2021)228] and school‐level research projects of the Yancheng Institute of Technology (xjr2022042). ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2475-4455 2475-4455 |
DOI: | 10.1002/pld3.564 |