A family of eukaryotic lysophospholipid acyltransferases with broad specificity

• Published in: FEBS letters Vol. 582; no. 2; pp. 305 - 309
• Main Authors: Ståhl, Ulf, Stålberg, Kjell, Stymne, Sten, Ronne, Hans
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 23.01.2008
• Subjects: 1-Acylglycerophosphocholine O-Acyltransferase - metabolism; Ale1; Animals; Arabidopsis; Base Sequence; Biologi; Biology; bovine serum albumin; BSA; DNA Primers; G3P; glycerol-3-phosphate; glycerol-3-phosphate acyltransferase; GPAT; Humans; LPA; LPAAT; LPC; LPCAT; LPE; LPEAT; LPG; LPGAT; LPI; LPIAT; LPLAT; LPS; LPSAT; lysophosphatidic acid; lysophosphatidic acid acyltransferase; lysophosphatidylcholine; lysophosphatidylcholine acyltransferase; lysophosphatidylethanolamine; lysophosphatidylethanolamine acyltransferase; lysophosphatidylglycerol; lysophosphatidylglycerol acyltransferase; lysophosphatidylinositol; lysophosphatidylinositol acyltransferase; lysophosphatidylserine; lysophosphatidylserine acyltransferase; Lysophospholipid acyltransferase; MBOAT protein; NATURAL SCIENCES; NATURVETENSKAP; phosphatidic acid; phosphatidylinositol; Phospholipid remodeling; Phylogeny; Saccharomyces cerevisiae; Slc1; Substrate Specificity; thin layer chromatography; TLC; wild-type; Yeast; LPE; Slc1; LPG; Yeast; LPI; G3P; TLC; LPS; BSA; LPSAT; WT; Phospholipid remodeling; LPEAT; LPGAT; LPAAT; Arabidopsis; LPCAT; Ale1; LPIAT; MBOAT protein; LPLAT; PA; Lysophospholipid acyltransferase; PI; LPA; GPAT; LPC
• ISSN: 0014-5793; 1873-3468; 1873-3468
• DOI: 10.1016/j.febslet.2007.12.020

The budding yeast ALE1 gene encodes a lysophospholipid acyltransferase (LPLAT) with broad specificity. We show that yeast LPLAT (ScLPLAT) belongs to a distinct protein family that includes human MBOAT1, MBOAT2, MBOAT4, and several closely related proteins from other eukaryotes. We further show that two plant proteins within this family, the Arabidopsis proteins AtLPLAT1 and AtLPLAT2, possess lysophospholipid acyltransferase activities similar to ScLPLAT. We propose that other members of this protein family, which we refer to as the LPLAT family, also are likely to possess LPLAT activity. Finally, we show that ScLPLAT differs from the specific lysophosphatidic acid acyltransferase that is encoded by SLC1 in that it cannot efficiently use lysophosphatidic acid produced by acylation of glycerol-3-phosphate in vitro.