Separation and Enrichment of Melamine in Feed by a New Molecularly Imprinted Solid-phase Extraction Column
[ Objective] The research aimed to develop a new molecular imprinting solid-phase extraction column for the separation and enrichment of melamine in feed. [ Method] The molecular imprinting polymer of melamine was prepared by mass polymerization method. This polymer was used as filler to prepare mol...
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Published in | Animal husbandry and feed science Vol. 6; no. 1; pp. 10 - 13 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Cranston
Wu Chu (USA-China) Science and Culture Media Corporation
01.02.2014
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Subjects | |
Online Access | Get full text |
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Summary: | [ Objective] The research aimed to develop a new molecular imprinting solid-phase extraction column for the separation and enrichment of melamine in feed. [ Method] The molecular imprinting polymer of melamine was prepared by mass polymerization method. This polymer was used as filler to prepare molecular imprinting polymer solid-phase extraction(MIP-SPE) column of melamine. The solid-phase extraction conditions were optimized and melamine content in concentrated solution was determined by using HPLC. [ Result] Melamine eould be separated selectively from feed and enriehed by self-made MIP-SPE column. It could wipe off complex matrix from feed and the addition recovery rate was 95%. The contrast experiment between MIP-SPE column and NMIP-SPE ( non-imprinted polymer- solid phase extraction) column showed that the extraction efficiency of the former was obviously higher than the latter. [ Conclusion] The home-made MIP- SPE column could be used to separate and enrich melamine in feed efficiently, with a broad application prospect. |
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Bibliography: | [ Objective] The research aimed to develop a new molecular imprinting solid-phase extraction column for the separation and enrichment of melamine in feed. [ Method] The molecular imprinting polymer of melamine was prepared by mass polymerization method. This polymer was used as filler to prepare molecular imprinting polymer solid-phase extraction(MIP-SPE) column of melamine. The solid-phase extraction conditions were optimized and melamine content in concentrated solution was determined by using HPLC. [ Result] Melamine eould be separated selectively from feed and enriehed by self-made MIP-SPE column. It could wipe off complex matrix from feed and the addition recovery rate was 95%. The contrast experiment between MIP-SPE column and NMIP-SPE ( non-imprinted polymer- solid phase extraction) column showed that the extraction efficiency of the former was obviously higher than the latter. [ Conclusion] The home-made MIP- SPE column could be used to separate and enrich melamine in feed efficiently, with a broad application prospect. Molecular imprinting; Solid-phase extraction ; Feed ; Melamine |
ISSN: | 1943-9911 |