mTORC1 and mTORC2 differentially promote natural killer cell development

• Published in: eLife Vol. 7
• Main Authors: Yang, Chao, Tsaih, Shirng-Wern, Lemke, Angela, Flister, Michael J, Thakar, Monica S, Malarkannan, Subramaniam
• Format: Journal Article
• Language: English
• Published: England eLife Sciences Publications Ltd 29.05.2018; eLife Sciences Publications, Ltd
• Subjects: Adaptive immunity; Animals; CD11b antigen; CD27 antigen; Clonal deletion; Cytotoxicity; Developmental stages; Female; Flow cytometry; FOXO1 protein; Gene expression; Homeostasis; Immunology; Immunology and Inflammation; Immunotherapy; Killer Cells, Natural - cytology; Killer Cells, Natural - immunology; Killer Cells, Natural - metabolism; Kinases; Lung Neoplasms - immunology; Lung Neoplasms - metabolism; Lung Neoplasms - secondary; Lymphocytes; Lymphoid cells; Male; Mechanistic Target of Rapamycin Complex 1 - genetics; Mechanistic Target of Rapamycin Complex 1 - metabolism; Mechanistic Target of Rapamycin Complex 2 - genetics; Mechanistic Target of Rapamycin Complex 2 - metabolism; Melanoma, Experimental - immunology; Melanoma, Experimental - metabolism; Melanoma, Experimental - pathology; Mice; Mice, Inbred C57BL; Mice, Knockout; Natural killer cells; NK cells; Rapamycin; Rapamycin-Insensitive Companion of mTOR Protein - physiology; Raptor; Regulatory-Associated Protein of mTOR - physiology; Rictor; Signal Transduction; T cell receptors; T-Box Domain Proteins - genetics; T-Box Domain Proteins - metabolism; TOR protein; Transcription factors; Tumor Cells, Cultured; United States > US; Rictor; mouse; NK cells; inflammation; immunology; Raptor
• ISSN: 2050-084X; 2050-084X
• DOI: 10.7554/elife.35619

Natural killer (NK) cells are innate lymphoid cells that are essential for innate and adaptive immunity. Mechanistic target of rapamycin (mTOR) is critical for NK cell development; however, the independent roles of mTORC1 or mTORC2 in regulating this process remain unknown. -mediated deletion of or in mice results in altered homeostatic NK cellularity and impaired development at distinct stages. The transition from the CD27 CD11b to the CD27 CD11b stage is impaired in cKO mice, while, the terminal maturation from the CD27 CD11b to the CD27 CD11b stage is compromised in cKO mice. Mechanistically, Raptor-deficiency renders substantial alteration of the gene expression profile including transcription factors governing early NK cell development. Comparatively, loss of Rictor causes more restricted transcriptome changes. The reduced expression of T-bet correlates with the terminal maturation defects and results from impaired mTORC2-Akt -FoxO1 signaling. Collectively, our results reveal the divergent roles of mTORC1 and mTORC2 in NK cell development.