Brassinosteroids control root epidermal cell fate via direct regulation of a MYB-bHLH-WD40 complex by GSK3-like kinases

In Arabidopsis, root hair and non-hair cell fates are determined by a MYB-bHLH-WD40 transcription factor complex and are regulated by many internal and environmental cues. Brassinosteroids play important roles in regulating root hair specification by unknown mechanisms. Here, we systematically exami...

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Published ineLife Vol. 3
Main Authors Cheng, Yinwei, Zhu, Wenjiao, Chen, Yuxiao, Ito, Shinsaku, Asami, Tadao, Wang, Xuelu
Format Journal Article
LanguageEnglish
Published England eLife Sciences Publications Ltd 25.04.2014
eLife Sciences Publications, Ltd
Subjects
Arabidopsis
brassinosteroid
Brassinosteroids
Cell division
Cell fate
Cloning
EGL3
Gene expression
Genetic analysis
Genetic engineering
GSK3-like kinase
Hair cells
Helix-loop-helix proteins (basic)
Kinases
Life sciences
Mutagenesis
Nicotiana benthamiana
Nuclei
Pattern formation
Phosphorylation
Plant Biology
Proteins
root epidermal cell fate
Transcription
Transcription factors
TTG1
Arabidopsis
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Summary:In Arabidopsis, root hair and non-hair cell fates are determined by a MYB-bHLH-WD40 transcription factor complex and are regulated by many internal and environmental cues. Brassinosteroids play important roles in regulating root hair specification by unknown mechanisms. Here, we systematically examined root hair phenotypes in brassinosteroid-related mutants, and found that brassinosteroid signaling inhibits root hair formation through GSK3-like kinases or upstream components. We found that with enhanced brassinosteroid signaling, GL2, a cell fate marker for non-hair cells, is ectopically expressed in hair cells, while its expression in non-hair cells is suppressed when BR signaling is reduced. Genetic analysis demonstrated that brassinosteroid-regulated root epidermal cell patterning is dependent on the WER-GL3/EGL3-TTG1 transcription factor complex. One of the GSK3-like kinases, BIN2, interacted with and phosphorylated EGL3, and EGL3s mutated at phosphorylation siteswere retained in hair cell nuclei. BIN2 phosphorylated TTG1 to inhibit the activity of the WER-GL3/EGL3-TTG1 complex. Thus, our study provides insights into the mechanism of brassinosteroid regulation of root hair patterning.
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ISSN:2050-084X
2050-084X
DOI:10.7554/elife.02525