Effects of combined extreme cold and drought stress on growth, photosynthesis, and physiological characteristics of cool-season grasses

• Published in: Scientific reports Vol. 14; no. 1; p. 116
• Main Authors: Li, Juanxia, Bai, Xiaoming, Ran, Fu, Zhang, Caizhong, Yan, Yubang, Li, Ping, Chen, Hui
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 02.01.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/158; 631/449; 704/172; Ascorbic acid; Carbon dioxide; Chlorophyll; Cold; Cold tolerance; Drought resistance; Electrical conductivity; Extreme cold; Germplasm; Glutathione reductase; Humanities and Social Sciences; Hydrogen peroxide; Hydroxyl radicals; L-Ascorbate peroxidase; Morphology; multidisciplinary; Photosynthesis; Physical characteristics; Physiology; Principal components analysis; Science; Science (multidisciplinary); Stomata; Stomatal conductance; Superoxide anions; Transpiration; Turf; Water use; Water use efficiency
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-49531-1

Abiotic stress is an important factor affecting turf establishment and limiting the sustainability of the turf industry. To alleviate the effects of combined cold and drought stress in cold- and drought-prone regions, it is essential to select and introduce turfgrass germplasms that are suitable for these conditions for successful turf establishment. Thus, we evaluated the effects of combined extreme cold and drought stress on the morphological, plant leaf functional, photosynthetic, and physiological and biochemical traits of 16 wild annual bluegrass ( Poa annua ) germplasms. We found that there were significant differences ( P  < 0.05) among different provenances, combined cold and drought stress, and the main interaction factors. Combined cold and drought stress altered the morphological characteristics of the 16 germplasms to varying degrees. Furthermore, combined cold and drought stress significantly reduced the net photosynthetic rate (Pn), stomatal conductance (gs), transpiration rate (Tr), instantaneous water use efficiency (WUE), chlorophyll content, chlorophyll fluorescence parameters, accumulated intercellular CO 2 concentration (Ci), and relative electrical conductivity (REC) and malondialdehyde (MDA), proline (Pro), soluble protein (SP), soluble sugar (SS), superoxide anion (O 2 .- ), hydrogen peroxide (H 2 O 2 ), and hydroxyl radical (·OH) and other active oxygen, and increased the superoxide dismutase activity (SOD), peroxidase activity (POD), catalase activity (CAT), ascorbate peroxidase activity (APX) and glutathione reductase (GR) activities. Comprehensive evaluation using principal component analysis (PCA), membership function analysis, and clustered heatmaps indicated that the ‘HZ’ germplasm had stronger combined cold and drought tolerance, whereas the ‘ZQ’ germplasm was more sensitive to combined cold and drought, which was roughly consistent with the order of morphological damage symptoms. Therefore, it is recommended to use the ‘HZ’ germplasm for planting projects in cold- and drought-prone areas, while the ‘ZQ’ germplasm is more suitable for use under warmer and non-water-deficient conditions.