A versatile genetic tool for post-translational control of gene expression in Drosophila melanogaster
Several techniques have been developed to manipulate gene expression temporally in intact neural circuits. However, the applicability of current tools developed for in vivo studies in is limited by their incompatibility with existing GAL4 lines and side effects on physiology and behavior. To circumv...
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Published in | eLife Vol. 6 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
England
eLife Sciences Publications Ltd
15.11.2017
eLife Sciences Publications, Ltd |
Subjects | |
Online Access | Get full text |
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Summary: | Several techniques have been developed to manipulate gene expression temporally in intact neural circuits. However, the applicability of current tools developed for in vivo studies in
is limited by their incompatibility with existing GAL4 lines and side effects on physiology and behavior. To circumvent these limitations, we adopted a strategy to reversibly regulate protein degradation with a small molecule by using a destabilizing domain (DD). We show that this system is effective across different tissues and developmental stages. We further show that this system can be used to control in vivo gene expression levels with low background, large dynamic range, and in a reversible manner without detectable side effects on the lifespan or behavior of the animal. Additionally, we engineered tools for chemically controlling gene expression (GAL80-DD) and recombination (FLP-DD). We demonstrate the applicability of this technology in manipulating neuronal activity and for high-efficiency sparse labeling of neuronal populations. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2050-084X 2050-084X |
DOI: | 10.7554/elife.30327 |