Olfactory Neuroepithelial Neural Progenitor Cells from Subjects with Bipolar I Disorder

Background: Research into the pathophysiology of bipolar disorder (BD) is limited by the inability to examine brain cellular processes in subjects with the illness. Methods: Endoscopic biopsy was performed in subjects with bipolar I disorder to establish olfactory neural progenitor (ONP) cell lines....

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Published inJournal of central nervous system disease Vol. 2017; no. 9; p. 117957351769452
Main Authors Gao, Yonglin, Winstead, Welby, Lei, Zhenmin, Lu, Chengliang, Roisen, Fred J., El-Mallakh, Rif S.
Format Journal Article
LanguageEnglish
Published London, England SAGE Publishing 01.01.2017
SAGE Publications
Sage Publications Ltd
Subjects
Authorship
Biopsy
Bipolar disorder
Brain research
Cell culture
Cell cycle
Cell lines
Fibroblasts
Glial fibrillary acidic protein
Medical research
Methodology
Myelin
Myelin basic protein
Nervous system
Nestin
Neural stem cells
Olfaction
Peer review
Peripherin
Pluripotency
Progenitor cells
Proteins
Stem cells
Studies
Tubulin
neural progenitors
olfactory neuroepithelium
bipolar disorder
Progenitor cells
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Summary:Background: Research into the pathophysiology of bipolar disorder (BD) is limited by the inability to examine brain cellular processes in subjects with the illness. Methods: Endoscopic biopsy was performed in subjects with bipolar I disorder to establish olfactory neural progenitor (ONP) cell lines. Olfactory function was assessed prebiopsy and postbiopsy using the University of Pennsylvania Smell Identification Test (UPSIT). Cells were characterized to determine their lineage. Results: There were no significant complications associated with the biopsy procedure, including olfaction. Outpatient olfactory neuroepithelial biopsy yielded ONP cells in three out of 13 biopsy attempts (23.1%). ONPs were positive for neuron-specific proteins (β-tubulin III, nestin, hexaribonucleotide binding protein-3, and peripherin) and glia-specific proteins (glial fibrillary acidic protein and myelin basic protein). Conclusions: ONP cells can be obtained safely from awake outpatients and are potentially useful for pathophysiological studies of bipolar illness and perhaps other neuropsychiatric conditions. Such cells allow for the investigation of potential pathological cellular processes without the confounding factors of genetic manipulation, which is required for induced pluripotent cells.
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This paper was subject to independent, expert peer review by a minimum of two blind peer reviewers. All editorial decisions were made by the independent academic editor. All authors have provided signed confirmation of their compliance with ethical and legal obligations including (but not limited to) use of any copyrighted material, compliance with ICMJE authorship and competing interests disclosure guidelines and, where applicable, compliance with legal and ethical guidelines on human and animal research participants.
ISSN:1179-5735
1179-5735
DOI:10.1177/1179573517694529