Citrimicrobium luteum gen. nov., sp. nov., aerobic anoxygenic phototrophic bacterium isolated from the gut of a sea cucumber Stichopus japonicus

• Published in: The journal of microbiology Vol. 52; no. 10; pp. 819 - 824
• Main Authors: Jung, Hong-Joo, Cha, In-Tae, Yim, Kyung June, Song, Hye Seon, Cho, Kichul, Kim, Daekyung, Lee, Hae-Won, Lee, Jae Kook, Seo, Myung-Ji, Roh, Seong Woon, Lee, Sung-Jae
• Format: Journal Article
• Language: English
• Published: Heidelberg The Microbiological Society of Korea 01.10.2014; Springer Nature B.V; 한국미생물학회
• Subjects: Aerobiosis; Alphaproteobacteria - classification; Alphaproteobacteria - genetics; Alphaproteobacteria - isolation & purification; Alphaproteobacteria - physiology; Animals; Apostichopus japonicus; Bacteria; Base Composition; Biomedical and Life Sciences; Carotenoids; catalase; Citromicrobium; Cluster Analysis; Cytosol - chemistry; digestive system; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Enzymes - analysis; Erythrobacter; fatty acids; Fatty Acids - analysis; Gastrointestinal Tract - microbiology; gelatin; genes; glycolipids; Glycolipids - analysis; Holothurioidea; Life Sciences; Lipids; Locomotion; Marine; Microbiology; Microscopy; Molecular Sequence Data; new genus; new species; nucleotide sequences; phospholipids; Phospholipids - analysis; photosynthetic bacteria; Phylogenetics; Phylogeny; Pigments, Biological - metabolism; polysorbates; Quinones - analysis; Republic of Korea; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; sequence homology; Sodium chloride; Sodium Chloride - metabolism; South Korea; Stichopus - microbiology; Stichopus japonicus; Taxonomy; Temperature; tyrosine; 생물학; Republic of Korea; United States > US; South Korea; Jeju Island; INW, Korea, Rep; gut bacterium; sea cucumber; polyphasic taxonomy
• ISSN: 1225-8873; 1976-3794; 1976-3794
• DOI: 10.1007/s12275-014-4136-7

A Gram-stain negative, yellow-pigmented, motile, pleomorphic bacterium, designated strain CBA4602 T , was isolated from the gut of the sea cucumber Stichopus japonicus , which was collected from Jeju Island in the Republic of Korea. In a phylogenetic analysis based on the 16S rRNA gene, strain CBA4602 T belonged to the order Sphingomonadales in the class Alphaproteobacteria . The 16S rRNA gene sequence similarity between strain CBA4602 T and ‘ Citromicrobium bathyomarinum ’ JF-1, the most closely related strain having nonvalidly published name, was 98.4%, followed by 95.2–96.7% identities with sequence of the other closest strains in the genus Erythrobacter . Strain CBA4602 T had bacteriochlorophyll a and carotenoids. Strain CBA4602 T grew in 0–10% (w/v) NaCl, at 10–42°C and pH 6.0–8.0, with optimal growth in 1–2% NaCl, at 30–37°C and pH 7.0. Strain CBA4602 T was positive for catalase and oxidase activities and was able to hydrolyse gelatine and Tween 20 and 40, but not starch, Tween 80 or l -tyrosine. The G+C content of genomic DNA from strain CBA4602 T was 68.0 mol% and Q-10 was the major detected isoprenoid quinone. The polar lipids were three unidentified phospholipids, three unidentified glycolipids, and two unidentified lipids. The dominant fatty acids were anteiso-C 15:0 , C 16:0 , anteiso-C 17:0 and C 18:0 . As considering the current taxonomic status of the genus ‘ Citromicrobium ’ and polyphasic taxonomic analyses, strain CBA4602 T represents a novel genus and species. The name Citrimicrobium luteum is proposed for the type strain CBA4602 T (=KACC 17668 T =JCM 19530 T ).