Within-subject reproducibility varies in multi-modal, longitudinal brain networks

• Published in: Scientific reports Vol. 13; no. 1; pp. 6699 - 18
• Main Authors: Nakuci, Johan, Wasylyshyn, Nick, Cieslak, Matthew, Elliott, James C., Bansal, Kanika, Giesbrecht, Barry, Grafton, Scott T., Vettel, Jean M., Garcia, Javier O., Muldoon, Sarah F.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 24.04.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 631/114/2408; 631/1647/245; 631/378/116/1925; Brain; Brain mapping; Brain Mapping - methods; EEG; Fingerprinting; Functional magnetic resonance imaging; Humanities and Social Sciences; Humans; Magnetic Resonance Imaging - methods; Magnetoencephalography; Magnetoencephalography - methods; multidisciplinary; Nerve Net; Nervous system; Neural networks; Neuroimaging; Reproducibility; Reproducibility of Results; Science; Science (multidisciplinary); Statistical analysis
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-33441-3

Network neuroscience provides important insights into brain function by analyzing complex networks constructed from diffusion Magnetic Resonance Imaging (dMRI), functional MRI (fMRI) and Electro/Magnetoencephalography (E/MEG) data. However, in order to ensure that results are reproducible, we need a better understanding of within- and between-subject variability over long periods of time. Here, we analyze a longitudinal, 8 session, multi-modal (dMRI, and simultaneous EEG-fMRI), and multiple task imaging data set. We first confirm that across all modalities, within-subject reproducibility is higher than between-subject reproducibility. We see high variability in the reproducibility of individual connections, but observe that in EEG-derived networks, during both rest and task, alpha-band connectivity is consistently more reproducible than connectivity in other frequency bands. Structural networks show a higher reliability than functional networks across network statistics, but synchronizability and eigenvector centrality are consistently less reliable than other network measures across all modalities. Finally, we find that structural dMRI networks outperform functional networks in their ability to identify individuals using a fingerprinting analysis. Our results highlight that functional networks likely reflect state-dependent variability not present in structural networks, and that the type of analysis should depend on whether or not one wants to take into account state-dependent fluctuations in connectivity.