LncRNA UCA1 promotes keratinocyte-driven inflammation via suppressing METTL14 and activating the HIF-1α/NF-κB axis in psoriasis

• Published in: Cell death & disease Vol. 14; no. 4; pp. 279 - 12
• Main Authors: Hu, Yibo, Lei, Li, Jiang, Ling, Zeng, Hongliang, Zhang, Yushan, Fu, Chuhan, Guo, Haoran, Dong, Yumeng, Ouyang, Yujie, Zhang, Xiaolin, Huang, Jinhua, Zeng, Qinghai, Chen, Jing
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 20.04.2023; Springer Nature B.V; Nature Publishing Group
• Subjects: 13/1; 13/109; 13/21; 13/51; 13/89; 14/32; 14/34; 45/90; 45/91; 631/250/262/2106/2518; 631/337/176/2016; 692/699/249/1313/1758; 82/58; Antibodies; Biochemistry; Biomedical and Life Sciences; Cell Biology; Cell Culture; Cell Proliferation; Cytokines; Cytokines - metabolism; Endothelial cells; Endothelial Cells - metabolism; Gene expression; Humans; Hypoxia-inducible factor 1a; Immunology; Inflammation; Inflammation - genetics; Inflammation - metabolism; Innate immunity; Keratinocytes; Keratinocytes - metabolism; Life Sciences; Methyltransferase; Methyltransferases - metabolism; Molecular modelling; N6-methyladenosine; NF-kappa B - genetics; NF-kappa B - metabolism; NF-κB protein; Non-coding RNA; Proteomes; Psoriasis; Psoriasis - pathology; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Signal transduction; Stat3 protein; Transcriptomes
• ISSN: 2041-4889; 2041-4889
• DOI: 10.1038/s41419-023-05790-4

Keratinocytes are closely associated with innate immunity and inflammatory responses, and are dysregulated during the development of psoriasis, but the underlying mechanisms are not yet fully understood. This work aims to reveal the effects of long non-coding RNA (lncRNA) UCA1 in psoriatic keratinocytes. UCA1 was identified as a psoriasis-related lncRNA that highly expressed in psoriatic lesions. The transcriptome and proteome data of keratinocyte cell line HaCaT showed that UCA1 could positively regulate inflammatory functions, such as response to cytokine. Furthermore, UCA1 silencing decreased inflammatory cytokine secretion and innate immunity gene expression in HaCaT, its culture supernatant also decreased the migration and tube formation ability of vascular endothelial cells (HUVECs). Mechanistically, UCA1 activated the NF-κB signaling pathway, which is regulated by HIF-1α and STAT3. We also observed a direct interaction between UCA1 and N6-methyladenosine (m 6 A) methyltransferase METTL14. Knocking down METTL14 counteracted the effects of UCA1 silencing, indicating that it can suppress inflammation. In addition, the levels of m 6 A-modified HIF-1α were decreased in psoriatic lesions, indicating that HIF-1α is a potential target of METTL14. Taken together, this work indicates that UCA1 positively regulates keratinocyte-driven inflammation and psoriasis development by binding to METTL14, and activating HIF-1α and NF-κB signaling pathway. Our findings provide new insights into the molecular mechanisms of keratinocyte-driven inflammation in psoriasis.