Hepatic lipocalin 2 promotes liver fibrosis and portal hypertension

• Published in: Scientific Reports Vol. 10; no. 1; p. 15558
• Main Authors: Chen, Jiegen, Argemi, Josepmaria, Odena, Gemma, Xu, Ming-Jiang, Cai, Yan, Massey, Veronica, Parrish, Austin, Vadigepalli, Rajanikanth, Altamirano, Jose, Cabezas, Joaquin, Gines, Pere, Caballeria, Juan, Snider, Natasha, Sancho-Bru, Pau, Akira, Shizuo, Rusyn, Ivan, Gao, Bin, Bataller, Ramon
• Format: Journal Article
• Language: English
• Published: London Springer Science and Business Media LLC 23.09.2020; Nature Publishing Group UK
• Subjects: 692/699/1503/1607; 692/699/1503/1607/1605; 692/699/1503/1607/1608; Animals; Carbon Tetrachloride; Carbon Tetrachloride - toxicity; Collagen Type I; Collagen Type I - genetics; Collagen Type I, alpha 1 Chain; Digestive System; Disease Models, Animal; Ethanol; Ethanol - toxicity; Female; Gastroenterology; Gene Expression Regulation; Gene Expression Regulation - genetics; Hepatitis, Alcoholic; Hepatitis, Alcoholic - blood; Hepatitis, Alcoholic - genetics; Hepatitis, Alcoholic - pathology; Hepatocytes; Hepatocytes - metabolism; Hepatocytes - pathology; Hipertensió portal; Humanities and Social Sciences; Humans; Hypertension, Portal; Hypertension, Portal - blood; Hypertension, Portal - genetics; Hypertension, Portal - pathology; Lipocalin-2; Lipocalin-2 - genetics; Lipocalins; Liver; Liver - metabolism; Liver - pathology; Liver Cirrhosis; Liver Cirrhosis - blood; Liver Cirrhosis - chemically induced; Liver Cirrhosis - genetics; Liver Cirrhosis - pathology; Liver diseases; Malalties del fetge; Male; Mice; Mice, Knockout; Microarray Analysis; Microarray Analysis - methods; multidisciplinary; Neutrophil Gelatinase Associated Lipocalin; Portal hypertension; Science; Science (multidisciplinary); Siderophores; Tissue Inhibitor of Metalloproteinase-1; Tissue Inhibitor of Metalloproteinase-1 - genetics
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-72172-7

Advanced fibrosis and portal hypertension influence short-term mortality. Lipocalin 2 (LCN2) regulates infection response and increases in liver injury. We explored the role of intrahepatic LCN2 in human alcoholic hepatitis (AH) with advanced fibrosis and portal hypertension and in experimental mouse fibrosis. We found hepatic LCN2 expression and serum LCN2 level markedly increased and correlated with disease severity and portal hypertension in patients with AH. In control human livers, LCN2 expressed exclusively in mononuclear cells, while its expression was markedly induced in AH livers, not only in mononuclear cells but also notably in hepatocytes. Lcn2 −/− mice were protected from liver fibrosis caused by either ethanol or CCl 4 exposure. Microarray analysis revealed downregulation of matrisome, cell cycle and immune related gene sets in Lcn2 −/− mice exposed to CCl 4 , along with decrease in Timp1 and Edn1 expression. Hepatic expression of COL1A1 , TIMP1 and key EDN1 system components were elevated in AH patients and correlated with hepatic LCN2 expressio n. In vitro , recombinant LCN2 induced COL1A1 expression. Overexpression of LCN2 increased HIF1A that in turn mediated EDN1 upregulation. LCN2 contributes to liver fibrosis and portal hypertension in AH and could represent a new therapeutic target.