Stripe Rust Effector Pst_9302 Inhibits Wheat Immunity to Promote Susceptibility
f. sp. is an obligate biotrophic fungus that causes destructive stripe rust disease in wheat. During infection, secretes virulence effectors via a specific infection structure-the haustorium-inside host cells to disturb host immunity and promote fungal colonization and expansion. Hence, the identifi...
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Published in | Plants (Basel) Vol. 13; no. 1; p. 94 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
27.12.2023
MDPI |
Subjects | |
Online Access | Get full text |
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Summary: | f. sp.
is an obligate biotrophic fungus that causes destructive stripe rust disease in wheat. During infection,
secretes virulence effectors via a specific infection structure-the haustorium-inside host cells to disturb host immunity and promote fungal colonization and expansion. Hence, the identification and functional analyses of
effectors are of great significance in deciphering the
pathogenicity mechanism. Here, we identified one candidate
effector Pst_9302 that could suppress Bax-triggered cell death in
. qRT-PCR analyses showed that the transcript levels of
were highly increased during the early infection stages of
. The transient expression of
in wheat via the type-three secretion system (T3SS) significantly inhibited the callose deposition induced by
EtHAn. During wheat-
interaction,
overexpression suppressed reactive oxygen species (ROS) accumulation and cell death caused by the avirulent
race CYR23. The host-induced gene silencing (HIGS) of
resulted in decreased
pathogenicity with reduced infection area. The results suggest that Pst_9302 plays a virulence role in suppressing plant immunity and promoting
pathogenicity. Moreover, wheat voltage-dependent anion channel 1 protein (TaVDAC1) was identified as candidate Pst_9302-interacting proteins by yeast two-hybrid (Y2H) screening. Pull-down assays using the His-Pst_9302 and GST-TaVDAC1 protein verified their interactions. These results suggest that Pst_9302 may modulate wheat TaVDAC1 to regulate plant immunity. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2223-7747 2223-7747 |
DOI: | 10.3390/plants13010094 |