IFT cargo and motors associate sequentially with IFT trains to enter cilia of C. elegans

• Published in: Nature communications Vol. 15; no. 1; p. 3456
• Main Authors: Mitra, Aniruddha, Loseva, Elizaveta, Peterman, Erwin J. G.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 24.04.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 14; 14/35; 14/63; 631/57/2265; 631/57/343/2281; 631/80/128/1383; 631/80/128/2343; 64/11; Animals; Axoneme - metabolism; Axoneme - ultrastructure; Biological Transport; Caenorhabditis elegans - metabolism; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - metabolism; Cargo; Chemoreception; Cilia; Cilia - metabolism; Cilia - ultrastructure; Dynein; Dyneins - metabolism; Flagella - metabolism; Flagella - ultrastructure; Humanities and Social Sciences; Imaging; Kinesin; Kinesins - genetics; Kinesins - metabolism; multidisciplinary; Particle tracking; Protein Transport; Proteins; Science; Science (multidisciplinary); Single Molecule Imaging; Tubulin; Tubulin - metabolism; Ultrastructure
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-024-47807-2

Intraflagellar transport (IFT) orchestrates entry of proteins into primary cilia. At the ciliary base, assembled IFT trains, driven by kinesin-2 motors, can transport cargo proteins into the cilium, across the crowded transition zone. How trains assemble at the base and how proteins associate with them is far from understood. Here, we use single-molecule imaging in the cilia of C. elegans chemosensory neurons to directly visualize the entry of kinesin-2 motors, kinesin-II and OSM-3, as well as anterograde cargo proteins, IFT dynein and tubulin. Single-particle tracking shows that IFT components associate with trains sequentially, both in time and space. Super-resolution maps of IFT components in wild-type and mutant worms reveal ciliary ultrastructure and show that kinesin-II is essential for axonemal organization. Finally, imaging cilia lacking kinesin-II and/or transition zone function uncovers the interplay of kinesin-II and OSM-3 in driving efficient transport of IFT trains across the transition zone. Intraflagellar transport is essential for the formation and function of cilia. Here, the authors use single-molecule imaging in live C. elegans to show that transport trains are formed by the sequential attachment of proteins before departing into the cilium.