Constructing bio-layer of heparin and type IV collagen on titanium surface for improving its endothelialization and blood compatibility

• Published in: Journal of materials science. Materials in medicine Vol. 27; no. 4; pp. 81 - 11
• Main Authors: Zhang, Kun, Chen, Jun-ying, Qin, Wei, Li, Jing-an, Guan, Fang-xia, Huang, Nan
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.04.2016; Springer Nature B.V
• Subjects: Animals; Biocompatibility; Biocompatible Materials; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Bionics; Cardiology; Ceramics; Chemistry and Materials Science; Collagen; Collagen Type IV - chemistry; Collagen Type IV - physiology; Composites; Construction; Dogs; Endothelial Cells - physiology; Femoral Artery; Glass; Heparin - chemistry; Heparin - physiology; Heparins; Humans; Materials Science; Materials Testing; Microscopy, Electron, Scanning; Natural Materials; Polymer Sciences; Regenerative Medicine/Tissue Engineering; Self assembly; Staining; Stents; Surface Properties; Surfaces and Interfaces; Surgical implants; Thin Films; Tissue Engineering Constructs and Cell Substrates; Titanium - chemistry; Titanium base alloys; Activate Partial Thromboplastin Time; Blood Compatibility; Platelet Adhesion Test; Fluid Flow Shear Stress; Collagen Coating
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-016-5693-6

The modification of cardiovascular stent surface for a better micro-environment has gradually changed to multi-molecule, multi-functional designation. In this study, heparin (Hep) and type IV collagen (IVCol) were used as the functional molecule to construct a bifunctional micro-environment of anticoagulation and promoting endothelialization on titanium (Ti). The surface characterization results (AFM, Alcian Blue 8GX Staining and fluorescence staining of IVCol) indicated that the bio-layer of Hep and IVCol were successfully fabricated on the Ti surface through electrostatic self-assembly. The APTT and platelet adhesion test demonstrated that the bionic layer possessed better blood compatibility compared with Ti surface. The adhesion, proliferation, migration and apoptosis tests of endothelial cells proved that the Hep/IVCol layer was able to enhance the endothelialization of the Ti surface. The in vivo animal implantation results manifested that the bionic surface could encourage new endothelialization. This work provides an important reference for the construction of multifunction micro-environment on the cardiovascular scaffold surface.