Laquinimod suppress antigen presentation in relapsing–remitting multiple sclerosis: In-vitro high-throughput gene expression study

Laquinimod (LAQ) is a new immunomodulatory drug shown to be effective in the treatment of relapsing–remitting multiple sclerosis (RRMS); however, its molecular target pathways are not well recognized. In this study we characterized in-vitro the molecular effects of LAQ in peripheral blood mononuclea...

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Bibliographic Details
Published inJournal of neuroimmunology Vol. 221; no. 1; pp. 87 - 94
Main Authors Gurevich, M., Gritzman, T., Orbach, R., Tuller, T., Feldman, A., Achiron, A.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 15.04.2010
Subjects
Adult
Allergy and Immunology
Antigen Presentation - drug effects
Antigens, CD - metabolism
Cell subpopulation
Cell Survival
Dose-Response Relationship, Drug
Female
Gene Expression Profiling - methods
Gene Expression Regulation - drug effects
Humans
Immunologic Factors - pharmacology
Killer Cells, Natural - drug effects
Laquinimod
Leukocytes, Mononuclear - drug effects
Leukocytes, Mononuclear - metabolism
Microarray gene expression
Middle Aged
Models, Immunological
Multiple sclerosis
Multiple Sclerosis, Relapsing-Remitting - immunology
Multiple Sclerosis, Relapsing-Remitting - pathology
Neurology
NF-kappaB-Inducing Kinase
Oligonucleotide Array Sequence Analysis - methods
PBMC
Protein Array Analysis - methods
Protein Serine-Threonine Kinases - metabolism
Quinolones - pharmacology
Microarray gene expression
Multiple sclerosis
Laquinimod
PBMC
Cell subpopulation
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Summary:Laquinimod (LAQ) is a new immunomodulatory drug shown to be effective in the treatment of relapsing–remitting multiple sclerosis (RRMS); however, its molecular target pathways are not well recognized. In this study we characterized in-vitro the molecular effects of LAQ in peripheral blood mononuclear cells (PBMC) of healthy subjects and RRMS patients by gene expression microarrays. We demonstrated that LAQ induced suppression of genes related to antigen presentation and corresponding inflammatory pathways. These findings were demonstrated mainly via the NFkB pathway. Analysis of PBMC subpopulations identified activation of Th2 response in CD14+ and CD4+ cells and suppression of proliferation in CD8+ cells.
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ISSN:0165-5728
1872-8421
1872-8421
DOI:10.1016/j.jneuroim.2010.02.010