Reactivity and prevalence of neutralizing antibodies against Japanese strains of bovine viral diarrhea virus subgenotypes

• Published in: Comparative immunology, microbiology and infectious diseases Vol. 34; no. 1; pp. 35 - 39
• Main Authors: Minami, Fujiko, Nagai, Makoto, Ito, Mika, Matsuda, Tatsuhiko, Takai, Hikaru, Jinkawa, Yoshiko, Shimano, Takeshi, Hayashi, Michiko, Seki, Yoshihisa, Sakoda, Yoshihiro, Sugiura, Katsuaki, Akashi, Hiroomi
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.01.2011; Elsevier
• Subjects: Allergy and Immunology; Animals; Antibodies, Neutralizing - metabolism; Antibodies, Viral - metabolism; Antigenic difference; Artiodactyla; Biological and medical sciences; Bovine viral diarrhea virus; Bovine Virus Diarrhea-Mucosal Disease - diagnosis; Bovine Virus Diarrhea-Mucosal Disease - epidemiology; Bovine Virus Diarrhea-Mucosal Disease - virology; Cattle; Cross Reactions - immunology; Diarrhea Viruses, Bovine Viral - classification; Diarrhea Viruses, Bovine Viral - genetics; Diarrhea Viruses, Bovine Viral - immunology; Epidemic virus; Fundamental and applied biological sciences. Psychology; Genetic Variation - genetics; Genetic Variation - immunology; Genotype; Infectious Disease; Japan - epidemiology; Microbiology; Miscellaneous; Neutralization test; Phylogeny; Prevalence; Subgenotype; Virology; Japan; Antigenic difference; Bovine viral diarrhea virus; Neutralization test; Epidemic virus; Subgenotype; Pestivirus; Epidemic; Prevalence; Zoopathogen; Epidemiology; Neutralizing antibody; Strain; Virus; Infection; Immunology; Bovine diarrhea virus; Flaviviridae; Veterinary
• ISSN: 0147-9571; 1878-1667
• DOI: 10.1016/j.cimid.2009.10.007

Abstract Bovine viral diarrhea virus (BVDV) field isolates show genetic and antigenic diversity. At least 14 subgenotypes of BVDV-1 and 4 of BVDV-2 have been identified in Artiodactyla worldwide. Of these, 6 subgenotypes of BVDV-1 and 1 of BVDV-2 have been isolated in Japan. Previously, we reported that each subgenotype virus expresses different antigenic characteristics. Here we investigated the reactivity of neutralizing antibodies against representative strains of Japanese BVDV subgenotypes using sera from 266 beef cattle to estimate the prevalence of this epidemic virus among cattle in Japan. Antibody titers at concentrations at least 4-fold higher than antibodies against other subgenotype viruses were considered subgenotype specific. Subgenotype-specific antibodies were detected from 117 (80.7%) of 145 sera samples (69.7% against BVDV-1a, 1.4% against BVDV-1b, 8.3% against BVDV-1c, and 1.4% against BVDV-2a). The results suggest that neutralization tests are useful in estimating currently epidemic subgenotypes of BVDV in the field.