Identification and analysis of isoflavone reductase gene family in Gossypium hirsutum L

• Published in: Scientific reports Vol. 13; no. 1; p. 5703
• Main Authors: Gui, Yanting, Fu, Guozhan, Li, Xuelin, Dai, Yinghao
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 07.04.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 631/208; 631/449; Chromosomes - metabolism; Drought; Evolutionary genetics; Gene expression; Gene Expression Regulation, Plant; Genes; Genome, Plant; Genomes; Gossypium - metabolism; Gossypium hirsutum; Hormones; Humanities and Social Sciences; Isoflavones; multidisciplinary; Multigene Family; Phylogeny; Physicochemical properties; Plant Proteins - genetics; Plant Proteins - metabolism; Regulatory Sequences, Nucleic Acid; Science; Science (multidisciplinary); Stress, Physiological - genetics
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-32213-3

Isoflavone reductase ( IFR ) is a key enzyme controlling isoflavone synthesis and widely involved in response to various stresses. In this study, the IFR genes in four Gossypium species and other 7 species were identified and analyzed in the whole genome, and the physicochemical properties, gene structures, cis -acting elements, chromosomal locations, collinearity relationships and expression patterns of IFR genes were systematically analyzed. 28, 28, 14 and 15 IFR genes were identified in Gossypium hirsutum , Gossypium barbadense , Gossypium arboreum and Gossypium raimondii , respectively, which were divided into five clades according to the evolutionary tree and gene structure. Collinear analysis showed that segmental duplication and whole genome duplication were the main driving forces in the process of evolution, and most genes underwent pure selection. Gene structure analysis showed that IFR gene family was relatively conserved. Cis -element analysis of promoter showed that most GhIFR genes contain cis -elements related to abiotic stresses and plant hormones. Analysis of GhIFR gene expression under different stresses showed that GhIFR genes were involved in the response to drought, salt, heat and cold stresses through corresponding network mechanisms, especially GhIFR9A . Phenotypic analysis after silencing GhIFR9A gene by VIGS was shown that GhIFR9A gene was involved in the response to salt stress. This study laid a foundation for the subsequent functional study of cotton IFR genes.