Multifunctional effects of Lactobacillus sakei HEM 224 on the gastrointestinal tract and airway inflammation

• Published in: Scientific reports Vol. 13; no. 1; pp. 17918 - 12
• Main Authors: Kim, Hye-Shin, Oh, Hanna, Kim, Bobae, Ji, Yosep, Holzapfel, Wilhelm H., Kang, Hyeji, Arellano-Ayala, Karina
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 20.10.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 631/326/2522; 692/700; Animal models; Animals; Antibiotic resistance; Colitis; Colitis - chemically induced; Colitis - therapy; Colon; Dextran; Dextran sulfate; Dextran Sulfate - toxicity; Disease Models, Animal; Gastrointestinal tract; Humanities and Social Sciences; Humans; Immunomodulation; Inflammation; Inflammatory bowel disease; Intestinal microflora; Intranasal administration; Lactobacillus sakei; Latilactobacillus sakei; Mice; Mice, Inbred C57BL; Microbiota; Mucosa; multidisciplinary; Oral administration; Ovalbumin; Probiotics; Respiratory System; Respiratory tract; Respiratory tract diseases; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-45043-0

Mucosal tissues serve as the first defense line and their commensal microbiota play a role in sustaining of host health. This study aimed to isolate and evaluate a putative probiotic strain on various mucosal regions. Lactobacillus sakei HEM 224 was isolated from traditional Korean kimchi and identified. In the safety assessment L. sakei HEM 224 showed negative results for hemolysis, biogenic amine production and transferable antibiotic resistance. The probiotic potential of strain HEM 224 in diverse mucosal areas was shown in two different models, viz. a murine model with colitis induced by dextran sulfate sodium (DSS) and an allergic airway inflammation model induced by ovalbumin (OVA). In the colitis model, oral administration of L. sakei HEM 224 improved colitis physiology with immunomodulation, enhancing barrier components and gut microbiota alteration. In the allergic airway inflammation model, the intranasal administration of the strain decreased type 2 inflammation and enhanced epithelial barrier integrity from the airways. These results demonstrate that L. sakei HEM 224 can ameliorate inflammatory conditions in both the gastrointestinal and respiratory tracts through the reinforcement of the epithelial barrier and immunomodulation.