Epigenetic modifications regulate cultivar-specific root development and metabolic adaptation to nitrogen availability in wheat

• Published in: Nature communications Vol. 14; no. 1; pp. 8238 - 15
• Main Authors: Zhang, Hao, Jin, Zhiyuan, Cui, Fa, Zhao, Long, Zhang, Xiaoyu, Chen, Jinchao, Zhang, Jing, Li, Yanyan, Li, Yongpeng, Niu, Yanxiao, Zhang, Wenli, Gao, Caixia, Fu, Xiangdong, Tong, Yiping, Wang, Lei, Ling, Hong-Qing, Li, Junming, Xiao, Jun
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 12.12.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 38/15; 38/23; 38/39; 38/90; 38/91; 631/449/2653; 631/449/2661; 631/449/711; Adaptation; Agronomy; Chromatin; Cultivars; Deoxyribonucleic acid; DNA; Epigenesis, Genetic; Epigenetics; Gene expression; Gene sequencing; Genes; Histone deacetylase; Histones; Histones - genetics; Histones - metabolism; Humanities and Social Sciences; Metabolism; Methyltransferase; multidisciplinary; Nitrogen; Nitrogen - metabolism; Nucleotide sequence; Plant Breeding; Plant growth; Root development; Science; Science (multidisciplinary); Sustainable agriculture; Transgenic plants; Triticum - metabolism; Wheat
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-023-44003-6

The breeding of crops with improved nitrogen use efficiency (NUE) is crucial for sustainable agriculture, but the involvement of epigenetic modifications remains unexplored. Here, we analyze the chromatin landscapes of two wheat cultivars (KN9204 and J411) that differ in NUE under varied nitrogen conditions. The expression of nitrogen metabolism genes is closely linked to variation in histone modification instead of differences in DNA sequence. Epigenetic modifications exhibit clear cultivar-specificity, which likely contributes to distinct agronomic traits. Additionally, low nitrogen (LN) induces H3K27ac and H3K27me3 to significantly enhance root growth in KN9204, while remarkably inducing NRT2 in J411. Evidence from histone deacetylase inhibitor treatment and transgenic plants with loss function of H3K27me3 methyltransferase shows that changes in epigenetic modifications could alter the strategy preference for root development or nitrogen uptake in response to LN. Here, we show the importance of epigenetic regulation in mediating cultivar-specific adaptation to LN in wheat. The analysis of wheat cultivars reveals that variations in histone modification, rather than DNA sequence, are closely linked to the expression of nitrogen metabolism genes and distinct agronomic traits. The findings suggest that epigenetic regulation plays a crucial role in cultivar-specific adaptation to low nitrogen conditions in wheat.