Overlapping Streptococcus pyogenes and Streptococcus dysgalactiae subspecies equisimilis household transmission and mobile genetic element exchange

• Published in: Nature communications Vol. 15; no. 1; p. 3477
• Main Authors: Xie, Ouli, Zachreson, Cameron, Tonkin-Hill, Gerry, Price, David J., Lacey, Jake A., Morris, Jacqueline M., McDonald, Malcolm I., Bowen, Asha C., Giffard, Philip M., Currie, Bart J., Carapetis, Jonathan R., Holt, Deborah C., Bentley, Stephen D., Davies, Mark R., Tong, Steven Y. C.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 24.04.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 45; 45/23; 631/114/2785; 692/308/174; 692/699/255/1318; Adolescent; Adult; Antimicrobial agents; Antimicrobial resistance; Australia; Bacteria; Cardiovascular disease; Child; Child, Preschool; Drug resistance; Drug Resistance, Bacterial - genetics; Epidemiology; Family Characteristics; Female; Gene transfer; Gene Transfer, Horizontal; Genes; Genome, Bacterial - genetics; Genomes; Genomic analysis; Homology; Horizontal transfer; Households; Humanities and Social Sciences; Humans; Impetigo; Infections; Interspersed Repetitive Sequences - genetics; Longitudinal Studies; Male; multidisciplinary; Native peoples; Pathogens; Phenotypes; Science; Science (multidisciplinary); Species; Streptococcal Infections - microbiology; Streptococcal Infections - transmission; Streptococcus - genetics; Streptococcus - isolation & purification; Streptococcus dysgalactiae; Streptococcus infections; Streptococcus pyogenes; Streptococcus pyogenes - classification; Streptococcus pyogenes - genetics; Streptococcus pyogenes - isolation & purification; Surveillance; Vaccines; Virulence; Young Adult; Australia
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-024-47816-1

Streptococcus dysgalactiae subspecies equisimilis (SDSE) and Streptococcus pyogenes share skin and throat niches with extensive genomic homology and horizontal gene transfer (HGT) possibly underlying shared disease phenotypes. It is unknown if cross-species transmission interaction occurs. Here, we conduct a genomic analysis of a longitudinal household survey in remote Australian First Nations communities for patterns of cross-species transmission interaction and HGT. Collected from 4547 person-consultations, we analyse 294 SDSE and 315 S. pyogenes genomes. We find SDSE and S. pyogenes transmission intersects extensively among households and show that patterns of co-occurrence and transmission links are consistent with independent transmission without inter-species interference. We identify at least one of three near-identical cross-species mobile genetic elements (MGEs) carrying antimicrobial resistance or streptodornase virulence genes in 55 (19%) SDSE and 23 (7%) S. pyogenes isolates. These findings demonstrate co-circulation of both pathogens and HGT in communities with a high burden of streptococcal disease, supporting a need to integrate SDSE and S. pyogenes surveillance and control efforts. Streptococcus dysgalactiae subsp. equisimilis is closely related to Streptococcus pyogenes and colonises the same sites in humans. This study examines cross-species transmission interactions and genetic exchange in a high disease burden setting.