Fate mapping analysis reveals a novel murine dermal migratory Langerhans-like cell population

• Published in: eLife Vol. 10
• Main Authors: Sheng, Jianpeng, Chen, Qi, Wu, Xiaoting, Dong, Yu Wen, Mayer, Johannes, Zhang, Junlei, Wang, Lin, Bai, Xueli, Liang, Tingbo, Sung, Yang Ho, Goh, Wilson Wen Bin, Ronchese, Franca, Ruedl, Christiane
• Format: Journal Article
• Language: English
• Published: England eLife Sciences Publications Ltd 26.03.2021; eLife Sciences Publications, Ltd
• Subjects: Antigen presentation; Antigen-presenting cells; Bone marrow; CD8 antigen; Cell fate; Cell migration; Contact dermatitis; dendritic cell; Dendritic cells; Dermis; Effector cells; Epidermis; Fate maps; Flow cytometry; Gene mapping; Genetic analysis; Haptens; Hypersensitivity; Immunological tolerance; Immunology and Inflammation; Langerhans cell; Langerhans cells; lymph node; Lymph nodes; Lymphatic system; Lymphocytes T; Osteoprogenitor cells; Population; skin; Skin diseases; mouse; Langerhans cell; inflammation; lymph node; cell migration; skin; immunology; dendritic cell; dermis
• ISSN: 2050-084X; 2050-084X
• DOI: 10.7554/elife.65412

Dendritic cells residing in the skin represent a large family of antigen-presenting cells, ranging from long-lived Langerhans cells (LC) in the epidermis to various distinct classical dendritic cell subsets in the dermis. Through genetic fate mapping analysis and single-cell RNA-sequencing, we have identified a novel separate population of LC-independent CD207 CD326 LC cells in the dermis that homed at a slow rate to the lymph nodes (LNs). These LC cells are long-lived and radio-resistant but, unlike LCs, they are gradually replenished by bone marrow-derived precursors under steady state. LC cells together with cDC1s are the main migratory CD207 CD326 cell fractions present in the LN and not, as currently assumed, LCs, which are barely detectable, if at all. Cutaneous tolerance to haptens depends on LC cells, whereas LCs suppress effector CD8 T-cell functions and inflammation locally in the skin during contact hypersensitivity. These findings bring new insights into the dynamism of cutaneous dendritic cells and their function opening novel avenues in the development of treatments to cure inflammatory skin disorders.