Genetic polymorphism of the thrombospondin-related apical merozoite protein (TRAMP) of Plasmodium knowlesi in Malaysia
Plasmodium knowlesi is a simian malaria parasite that causes significant zoonotic infections in Southeast Asia, particularly in Malaysia. The Plasmodium thrombospondin-related apical merozoite protein (TRAMP) plays an essential role in the invasion of the parasite into its host erythrocyte. The pr...
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Published in | Parasitology research (1987) Vol. 122; no. 1; pp. 195 - 200 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.01.2023
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: |
Plasmodium knowlesi
is a simian malaria parasite that causes significant zoonotic infections in Southeast Asia, particularly in Malaysia. The
Plasmodium
thrombospondin-related apical merozoite protein (TRAMP) plays an essential role in the invasion of the parasite into its host erythrocyte. The present study investigated the genetic polymorphism and natural selection of the full length
PkTRAMP
from
P. knowlesi
clinical isolates from Malaysia. Blood samples (
n
= 40) were collected from
P. knowlesi
malaria patients from Peninsular Malaysia and Malaysian Borneo. The
PkTRAMP
gene was amplified using PCR, followed by cloning into a plasmid vector and sequenced. Results showed that the nucleotide diversity of
PkTRAMP
was low (π: 0.009).
Z
-test results indicated negative (purifying) selection of
PkTRAMP
. The alignment of the deduced amino acid sequences of PkTRAMP of Peninsular Malaysia and Malaysian Borneo revealed 38 dimorphic sites. A total of 27 haplotypes were identified from the amino acid sequence alignment. Haplotype analysis revealed that there was no clustering of PkTRAMP from Peninsular Malaysia and Malaysian Borneo. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Handling Editor: Una Ryan |
ISSN: | 0932-0113 1432-1955 |
DOI: | 10.1007/s00436-022-07716-z |