The natural plant stress elicitor cis-jasmone causes cultivar-dependent reduction in growth of the stink bug, Euschistus heros and associated changes in flavonoid concentrations in soybean, Glycine max

• Published in: Phytochemistry (Oxford) Vol. 131; pp. 84 - 91
• Main Authors: da Graça, José P., Ueda, Tatiana E., Janegitz, Tatiani, Vieira, Simone S., Salvador, Mariana C., de Oliveira, Maria C.N., Zingaretti, Sonia M., Powers, Stephen J., Pickett, John A., Birkett, Michael A., Hoffmann-Campo, Clara B.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.11.2016; Elsevier
• Subjects: Animals; cis-jasmone; Cyclopentanes - pharmacology; Defence; Development; Euschistus heros; Feeding Behavior - drug effects; Flavonoid; Flavonoids - analysis; Genistein - analysis; Glucosides - analysis; Glycine max; Glycine max - chemistry; Glycine max - parasitology; Heteroptera - drug effects; Isoflavones - analysis; Oxylipins - pharmacology; Seeds - chemistry; cis-jasmone; Development; Euschistus heros; Glycine max; Flavonoid; Defence
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/j.phytochem.2016.08.013

To test the hypothesis that the plant stress related elicitor cis-jasmone (cJ) provides protection in soybean pods against the seed-sucking stink bug pest, Euschistus heros, the growth of E. heros on cJ-treated pods was investigated using three soybean cultivars differing in insect susceptibility, i.e. BRS 134 (susceptible), IAC 100 (resistant) and Dowling (resistant). E. heros showed reduced weight gain when fed cJ-treated Dowling, whereas no effect on weight gain was observed when fed other treated cultivars. Using analysis of variance, a three factor (cultivar x treatment x time) interaction was observed with concentrations of the flavonoid glycosides daidzin and genistin, and their corresponding aglycones, daidzein and genistein. There were increases in genistein and genistin concentrations in cJ-treated Dowling at 144 and 120 h post treatment, respectively. Higher concentrations of malonyldaidzin and malonylgenistin in Dowling, compared to BRS 134 and IAC 100, were observed independently of time, the highest concentrations being observed in cJ-treated seeds. Levels of glycitin and malonylglycitin were higher in BRS 134 and IAC 100 compared to Dowling. Canonical variate analysis indicated daidzein (in the first two canonical variates) and genistein (in the first only) as important discriminatory variables. These results suggest that cJ treatment leads to an increase in the levels of potentially defensive isoflavonoids in immature soybean seeds, but the negative effect upon E. heros performance is cultivar-dependent. cis-Jasmone treatment of soybean pods changes flavonoid levels and negatively affects Euschistus heros growth in a cultivar dependent manner. [Display omitted] •cis-Jasmone (cJ) affects levels of defence flavonoids in immature seeds of soybean, Glycine max.•The weight gain of the seed sucking stink bug pest, Euschistus heros, is reduced when fed on cJ-treated cv. Dowling only.•The negative effect of cJ treatment upon E. heros performance is cultivar dependent.