Effects of calmodulin on expression of lignin-modifying enzymes in Pleurotus ostreatus
Previously, we suppressed the expression of genes encoding isozymes of lignin peroxidase (LiP) and manganese peroxidase (MnP) using a calmodulin (CaM) inhibitor, W7, in the white-rot fungus Phanerochaete chrysosporium ; this suggested that CaM positively regulates their expression. Here, we studied...
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Published in | Current genetics Vol. 61; no. 2; pp. 127 - 140 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.05.2015
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Previously, we suppressed the expression of genes encoding isozymes of lignin peroxidase (LiP) and manganese peroxidase (MnP) using a calmodulin (CaM) inhibitor, W7, in the white-rot fungus
Phanerochaete chrysosporium
; this suggested that CaM positively regulates their expression. Here, we studied the role of CaM in another white-rot fungus,
Pleurotus ostreatus
, which produces MnP and versatile peroxidase (VP), but not LiP. W7 upregulated Mn
2+
-dependent oxidation of guaiacol, suggesting that CaM negatively regulates the production of the enzymes. Suppression of
CaM
in
P. ostreatus
using RNAi also led to upregulation of enzyme activity, whereas overexpression of
CaM
in
P. ostreatus
caused downregulation. Real-time RT-PCR showed that
MnP1
-
6
and
VP3
levels in the
CaM
-knockdown strain were higher than those in the wild-type strain, while
MnP
-
5 and
-
6
and
VP1 and 2
levels in the
CaM
-overexpressing strain were lower than in the wild type. Moreover, we also found that another ligninolytic enzyme, laccase, which is not produced by
P. chrysosporium
, was negatively regulated by CaM in
P. ostreatus
similar to MnP and VP. Although overexpression of
CaM
did not reduce the ability of
P. ostreatus
to digest beech wood powder, the percentage of lignin remaining in the digest was slightly higher than in the wild-type strain digest. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0172-8083 1432-0983 |
DOI: | 10.1007/s00294-014-0460-z |