Transfection types, methods and strategies: a technical review

Transfection is a modern and powerful method used to insert foreign nucleic acids into eukaryotic cells. The ability to modify host cells' genetic content enables the broad application of this process in studying normal cellular processes, disease molecular mechanism and gene therapeutic effect...

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Published inPeerJ (San Francisco, CA) Vol. 9; p. e11165
Main Authors Chong, Zhi Xiong, Yeap, Swee Keong, Ho, Wan Yong
Format Journal Article
LanguageEnglish
Published United States PeerJ, Inc 21.04.2021
PeerJ Inc
Subjects
Acids
Adenoviruses
Biotechnology
Cell Biology
Chemicals
Controls
Cytotoxicity
Decision making
Efficiency
Gene therapy
Genetic engineering
Genomes
Handbooks
Herpes viruses
Methods
Molecular Biology
Mutagenesis
Nucleic acids
Plasmids
Reagents
Reviews
Transfection
Vectors (Biology)
Viral infections
Mali
Controls
Transfection
Nucleic acids
Efficiency
Chemicals
Methods
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Summary:Transfection is a modern and powerful method used to insert foreign nucleic acids into eukaryotic cells. The ability to modify host cells' genetic content enables the broad application of this process in studying normal cellular processes, disease molecular mechanism and gene therapeutic effect. In this review, we summarized and compared the findings from various reported literature on the characteristics, strengths, and limitations of various transfection methods, type of transfected nucleic acids, transfection controls and approaches to assess transfection efficiency. With the vast choices of approaches available, we hope that this review will help researchers, especially those new to the field, in their decision making over the transfection protocol or strategy appropriate for their experimental aims.
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ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.11165