Bridging of host-microbiota tryptophan partitioning by the serotonin pathway in fungal pneumonia

• Published in: Nature communications Vol. 14; no. 1; pp. 5753 - 21
• Main Authors: Renga, Giorgia, D’Onofrio, Fiorella, Pariano, Marilena, Galarini, Roberta, Barola, Carolina, Stincardini, Claudia, Bellet, Marina M., Ellemunter, Helmut, Lass-Flörl, Cornelia, Costantini, Claudio, Napolioni, Valerio, Ehrlich, Allison K., Antognelli, Cinzia, Fini, Massimo, Garaci, Enrico, Nunzi, Emilia, Romani, Luigina
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 16.09.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 14/34; 45/41; 45/77; 45/90; 631/326/2565/2134; 64/60; 692/699/1785/4039; 692/699/255/1672; 82/1; 96/106; 96/21; 96/63; Amino acids; Animals; Aspergillosis; Cystic fibrosis; Dioxygenase; Homeostasis; Humanities and Social Sciences; Humans; Immune clearance; Inflammation; Influenza, Human; Intestinal microflora; Intestine; Lungs; Mast cells; Metabolism; Metabolites; Mice; Microbiota; Microorganisms; Mood; multidisciplinary; Mycoses; Partitioning; Pneumonia; Science; Science (multidisciplinary); Serotonin; Tryptophan
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-023-41536-8

The aromatic amino acid L-tryptophan (Trp) is essentially metabolized along the host and microbial pathways. While much is known about the role played by downstream metabolites of each pathways in intestinal homeostasis, their role in lung immune homeostasis is underappreciated. Here we have examined the role played by the Trp hydroxylase/5-hydroxytryptamine (5-HT) pathway in calibrating host and microbial Trp metabolism during Aspergillus fumigatus pneumonia. We found that 5-HT produced by mast cells essentially contributed to pathogen clearance and immune homeostasis in infection by promoting the host protective indoleamine-2,3-dioxygenase 1/kynurenine pathway and limiting the microbial activation of the indole/aryl hydrocarbon receptor pathway. This occurred via regulation of lung and intestinal microbiota and signaling pathways. 5-HT was deficient in the sputa of patients with Cystic fibrosis, while 5-HT supplementation restored the dysregulated Trp partitioning in murine disease. These findings suggest that 5-HT, by bridging host-microbiota Trp partitioning, may have clinical effects beyond its mood regulatory function in respiratory pathologies with an inflammatory component. Serotonin regulates mood as well as intestinal homeostasis, but its role in lung immune homeostasis is less clear. Here, Renga et al. show that serotonin regulates immune and microbial metabolic functions in respiratory pneumonia, beyond its mood regulatory function, by modulating tryptophan metabolism in the cystic fibrosis lung.