Expression and significance of glucose transporter-1, P-glycoprotein, multidrug resistance-associated protein and glutathione S-transferase-π in laryngeal carcinoma

• Published in: Oncology letters Vol. 9; no. 2; pp. 806 - 810
• Main Authors: MAO, ZHONG-PING, ZHAO, LI-JUN, ZHOU, SHUI-HONG, LIU, MENG-QIN, TAN, WEI-FENG, YAO, HONG-TIAN
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.02.2015; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: Biotechnology; Cancer; Chemotherapy; Development and progression; Gene expression; Genetic aspects; Genetic research; Glucose; glucose transporter-1; glutathione-s-transferase-π; Hypoxia; Immunoglobulins; Laryngeal cancer; laryngeal carcinoma; Medical prognosis; Metastasis; multidrug resistance-associated protein; Multidrug resistant organisms; Multivariate analysis; Oncology, Experimental; P-glycoprotein; Properties; Proteins; Radiation therapy; Rodents; Survival analysis; Tumor proteins; China; P-glycoprotein; glutathione-s-transferase-π; glucose transporter-1; laryngeal carcinoma; multidrug resistance-associated protein
• ISSN: 1792-1074; 1792-1082
• DOI: 10.3892/ol.2014.2752

Increasing glucose transporter-1 (GLUT-1) activity is one of the most important ways to increase the cellular influx of glucose. We previously demonstrated that increased GLUT-1 expression was an independent predictor of survival in patients with laryngeal carcinoma. Thus, GLUT-1 may present a novel therapeutic target in laryngeal carcinoma. In this study, the expression of GLUT-1, P-glycoprotein (P-gp), multidrug resistance-associated protein 1 (MRP1) and glutathione S-transferase-π (GST-π) in laryngeal carcinomas was investigated by immunohistochemistry. Additionally, possible correlations between GLUT-1 and P-gp, MRP1 and GST-π and various clinicopathological parameters were analyzed. In this study, 52.9% (18/34), 58.8% (20/34), 20.6% (7/34) and 58.8% (20/34) of the laryngeal carcinomas were positive for GLUT-1, P-gp, MRP1 and GST-π, respectively. The expression of GLUT-1, P-gp, MRP1 and GST-π was higher in laryngeal carcinoma specimens when compared with laryngeal precancerous lesions (P<0.05). Pearson's correlation analysis showed correlations between GLUT-1 and P-gp (r=0.364; P=0.034), GLUT-1 and MRP1 (r=0.359; P=0.037) and P-gp and GST-π (r=0.426; P=0.012). GLUT-1 expression was found to significantly correlate with tumor-node-metastasis classification (P=0.02) and clinical stage (P=0.037). Furthermore, P-gp was found to significantly correlate with clinical stage (P=0.026). Univariate analysis showed that MRP1 expression was significantly associated with poor survival (c2=5.16; P=0.023). Multivariate analysis revealed that lymph node metastasis (P=0.009) and MRP1 overexpression (P=0.023) were significant predictors of poor survival. In the present study, the expression of GLUT-1, P-gp, MRP1 and GST-π in laryngeal carcinomas was investigated, as well as the correlations between these proteins. P-gp was found to significantly correlate with clinical stage, while MRP1 overexpression was significantly associated with poor survival.