Predictive model for growth of Bacillus cereus during cooling of cooked rice

• Published in: International journal of food microbiology Vol. 290; pp. 49 - 58
• Main Authors: Juneja, Vijay K., Golden, Chase E., Mishra, Abhinav, Harrison, Mark A., Mohr, Tim, Silverman, Meryl
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 02.02.2019; Elsevier BV
• Subjects: agar; ambient temperature; Bacillus cereus; Baranyi model; cold treatment; Cooling; Cooling rate; Dynamic models; egg yolk; Food safety; Foodborne diseases; Foodborne pathogens; Goodness of fit; growth models; Growth rate; logit analysis; Mannitol; Outbreaks; polymyxins; prediction; Prediction models; Predictive microbiology; Rice; Spores; Statistical analysis; Statistical tests; storage temperature; Temperature profiles; Yolk; Foodborne pathogens; Food safety; Predictive microbiology; Baranyi model
• ISSN: 0168-1605; 1879-3460; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2018.09.023

Bacillus cereus is frequently implicated in foodborne outbreaks associated with the consumption of cooked rice. The main contributing factors leading to outbreaks is rice cooked in large quantities and subsequently, inadequately chilled or stored at room temperatures for a prolonged period of time prior to consumption. Bacillus cereus growth in cooked rice inoculated with approximately 2 log CFU/g of heat-shocked (80 °C/10 min) spores at several isothermal conditions (between 10 and 49 °C) was quantified. B. cereus populations were determined by plating on mannitol egg yolk polymyxin agar and incubating at 30 °C for 24 h. Primary growth models, namely Baranyi, Huang, modified Gompertz, and logistic models were fitted to growth data. Specific growth rates from all four primary models were used to fit the modified Ratkowsky square-root model with respect to temperature. All four primary models were well fitted by the modified Ratkowsky model (R2 values from 0.90–0.99). Based on the goodness of fit secondary model statistics (R2, SSE, RMSE), the Baranyi model performed the best and was chosen for tertiary modeling. Acceptable prediction zone (APZ) analysis was performed for validation of the Baranyi model predictions during single rate exponential and biphasic linear cooling temperature profiles. For single rate cooling, 23 of the 24 predictions fell within the APZ (−1.0 to 0.5 log CFU/g). For biphasic linear cooling, 26 of the 28 predictions fell within the APZ. The developed dynamic model can be used to predict potential B. cereus growth from spores in cooked rice during chilling and thus, support the disposition of product subject to cooling deviations. •We investigated the growth kinetics of Bacillus cereus in cooked rice.•Four different growth models were used to estimate the growth in cooked rice.•A dynamic model to estimate growth was developed.•The model will assist the food industry to evaluate risk of B. cereus growth in cooked rice.