Bovine herpesvirus 1: immune responses in mice and cattle injected with plasmid DNA

• Published in: Journal of Virology Vol. 67; no. 9; pp. 5664 - 5667
• Main Authors: Cox, G.J.M, Zamb, T.J, Babiuk, L.A
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.09.1993
• Subjects: ADN; Animals; Antibodies, Viral - blood; Antibody Formation; ANTIGENE; ANTIGENOS; BOVIN; Cattle; DNA, Viral - immunology; Enzyme-Linked Immunosorbent Assay; GANADO BOVINO; GENE; GENES; Genes, Viral; GLICOPROTEINAS; GLYCOPROTEINE; Herpesviridae - immunology; HERPESVIRUS BOVIN; HERPESVIRUS BOVINO; INJECTION; INYECCION; Mice; Plasmids; PROTEINAS; PROTEINE; RATON; REACCION ANTIGENO-ANTICUERPO; REACTION ANTIGENE ANTICORPS; REPONSE IMMUNITAIRE; RESPUESTA INMUNOLOGICA; SOURIS; VACCIN SYNTHETIQUE; VACCINATION; Vaccines, Synthetic - immunology; VACUNA SINTETICA; VACUNACION; Viral Proteins - genetics; Viral Proteins - immunology; Viral Vaccines - immunology; VIRUS DE LOS ANIMALES; VIRUS DES ANIMAUX
• ISSN: 0022-538X; 1098-5514
• DOI: 10.1128/JVI.67.9.5664-5667.1993

Mice and cattle injected with plasmids encoding bovine herpesvirus 1 (BHV-1) glycoproteins developed gene-specific antibody responses capable of neutralizing BHV-1. The ability of animals to respond serologically to DNA injections was in part dependent on the quantity of DNA injected and was also negatively affected by carrier DNA. Calves injected with a plasmid encoding BHV-1 gIV developed significant antibody titers to gIV and shed less virus than did the control calf after challenge. This report indicates the potential of DNA injection as a method of vaccination