Spatially resolved single-cell genomics and transcriptomics by imaging

The recent advent of genome-scale imaging has enabled single-cell omics analysis in a spatially resolved manner in intact cells and tissues. These advances allow gene expression profiling of individual cells, and hence in situ identification and spatial mapping of cell types, in complex tissues. The...

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Bibliographic Details
Published inNature methods Vol. 18; no. 1; pp. 18 - 22
Main Author Zhuang, Xiaowei
Format Journal Article
LanguageEnglish
Published New York Nature Publishing Group US 01.01.2021
Nature Publishing Group
Subjects
631/1647
631/61
Bioinformatics
Biological Microscopy
Biological Techniques
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Comment
Comparative analysis
Computational Biology - methods
Gene expression
Gene Expression Profiling
Gene mapping
Genetic transcription
Genomes
Genomics
Genomics - methods
Humans
Life Sciences
Methods
Multimodal Imaging - methods
Proteome - analysis
Proteomics
Regulatory mechanisms (biology)
Single-Cell Analysis - methods
Software
Spatial Analysis
Spatial discrimination
Spatial resolution
Transcriptome
Transcriptomes
United States
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Summary:The recent advent of genome-scale imaging has enabled single-cell omics analysis in a spatially resolved manner in intact cells and tissues. These advances allow gene expression profiling of individual cells, and hence in situ identification and spatial mapping of cell types, in complex tissues. The high spatial resolution of these approaches further allows determination of the spatial organizations of the genome and transcriptome inside cells, both of which are key regulatory mechanisms for gene expression.
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ISSN:1548-7091
1548-7105
1548-7105
DOI:10.1038/s41592-020-01037-8