Multi-crosslinking hydrogels with robust bio-adhesion and pro-coagulant activity for first-aid hemostasis and infected wound healing

• Published in: Bioactive materials Vol. 16; pp. 388 - 402
• Main Authors: Zou, Chen-Yu, Lei, Xiong-Xin, Hu, Juan-Juan, Jiang, Yan-Lin, Li, Qian-Jin, Song, Yu-Ting, Zhang, Qing-Yi, Li-Ling, Jesse, Xie, Hui-Qi
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.10.2022; KeAi Publishing; KeAi Communications Co., Ltd
• Subjects: Bio-adhesion; First-aid hemostasis; Infected wound healing; Polysaccharide-based hydrogel; Tannic acid; Tannic acid; Polysaccharide-based hydrogel; Infected wound healing; Bio-adhesion; First-aid hemostasis
• ISSN: 2452-199X; 2452-199X
• DOI: 10.1016/j.bioactmat.2022.02.034

Bio-adhesive polysaccharide-based hydrogels have attracted much attention in first-aid hemostasis and wound healing for excellent biocompatibility, antibacterial property and pro-healing bioactivity. Yet, the inadequate mechanical properties and bio-adhesion limit their applications. Herein, based on dynamic covalent bonds, photo-triggered covalent bonds and hydrogen bonds, multifunctional bio-adhesive hydrogels comprising modified carboxymethyl chitosan, modified sodium alginate and tannic acid are developed. Multi-crosslinking strategy endows hydrogels with improved strength and flexibility simultaneously. Owing to cohesion enhancement strategy and self-healing ability, considerable bio-adhesion is presented by the hydrogel with a maximal adhesion strength of 162.6 kPa, 12.3-fold that of commercial fibrin glue. Based on bio-adhesion and pro-coagulant activity (e.g., the stimulative aggregation and adhesion of erythrocytes and platelets), the hydrogel reveals superior hemostatic performance in rabbit liver injury model with blood loss of 0.32 g, only 54.2% of that in fibrin glue. The healing efficiency of hydrogel for infected wounds is markedly better than commercial EGF Gel and Ag+ Gel due to the enhanced antibacterial and antioxidant properties. Through the multi-crosslinking strategy, the hydrogels show enhanced mechanical properties, fabulous bio-adhesion, superior hemostatic performance and promoting healing ability, thereby have an appealing application value for the first-aid hemostasis and infected wound healing. [Display omitted] •The multifunctional hydrogel comprising polysaccharides and tannic acid was developed through multi-crosslinking strategy.•The multifunctional hydrogel showed 12.3-fold adhesion strength than commercial fibrin glue.•The multifunctional hydrogel revealed pro-coagulant activity and excellent hemostatic effect in vivo.•The multifunctional hydrogel effectively promoted the healing of infected wounds via multiple mechanisms.