Nondefective rotavirus mutants with an NSP1 gene which has a deletion of 500 nucleotides, including a cysteine-rich zinc finger motif-encoding region (Nucleotides 156 to 248), or which has a nonsense codon at nucleotides 153 to 155

We isolated two nondefective bovine rotavirus mutants (A5-10 and A5-16 clones) which have nonsense mutations in the early portion of the open reading frame of the NSP1 gene. In the NSP1 gene (1,587 bases long) of A5-10, a nonsense codon is present at nucleotides 153 to 155 just upstream of the codin...

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Bibliographic Details
Published inJournal of Virology Vol. 70; no. 6; pp. 4125 - 4130
Main Authors Taniguchi, K. (Sapporo Medical University School of Medicine, Sapporo, Japan.), Kojima, K, Urasawa, S
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.06.1996
Subjects
Amino Acid Sequence
Animals
Base Sequence
Cattle
CLONE
CLONES
CODE GENETIQUE
CODIGO GENETICO
Codon, Nonsense
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
CULTIVO DE CELULAS
CULTURE DE CELLULE
EXPRESION GENICA
EXPRESSION DES GENES
GENE
Gene Deletion
GENES
Genes, Viral
Molecular Sequence Data
MUTACION
MUTANT
MUTANTES
MUTATION
PROTEINAS
PROTEINE
ROTAVIRUS
Rotavirus - genetics
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
SINTESIS DE PROTEINAS
SYNTHESE PROTEIQUE
Viral Nonstructural Proteins - genetics
VIRUS DE LOS ANIMALES
VIRUS DES ANIMAUX
Zinc Fingers - genetics
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Summary:We isolated two nondefective bovine rotavirus mutants (A5-10 and A5-16 clones) which have nonsense mutations in the early portion of the open reading frame of the NSP1 gene. In the NSP1 gene (1,587 bases long) of A5-10, a nonsense codon is present at nucleotides 153 to 155 just upstream of the coding region (nucleotides 156 to 230) of a cysteine-rich Zn finger motif. A5-16 gene 5 (1,087 bases long) was found to have a large deletion of 500 bases corresponding to nucleotides 142 to 641 of a parent A5-10 NSP1 gene and to have a nonsense codon at nucleotides 183 to 185, which resulted from the deletion. Expression of gene 5-specific NSP1 could not be detected in MA-104 cells infected with the A5-10 or A5-16 clone or in an in vitro translation system using the plasmids with gene 5 cDNA from A5-10 or A5-16. Nevertheless, both A5-10 and A5-16 replicated well in cultured cells, although the plaque size of A5-16 was extremely small
Bibliography:9620131
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ISSN:0022-538X
1098-5514
DOI:10.1128/JVI.70.6.4125-4130.1996