Effect of S53P4 bone substitute on staphylococcal adhesion and biofilm formation on other implant materials in normal and hypoxic conditions

• Published in: Journal of materials science. Materials in medicine Vol. 26; no. 9; pp. 239 - 10
• Main Authors: Pérez-Tanoira, R., García-Pedrazuela, M., Hyyrynen, T., Soininen, A., Aarnisalo, A., Nieminen, Mikko T., Tiainen, V.-M., Konttinen, Y. T., Kinnari, T. J.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.09.2015; Springer Nature B.V
• Subjects: Adhesion; Bacteria; Bacterial Adhesion - drug effects; Biocompatibility; Biocompatibility Studies; Biofilms; Bioglass; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Bone Substitutes - pharmacology; Ceramics; Chemistry and Materials Science; Colony Count, Microbial; Composites; Formations; Glass; Hydrogen-Ion Concentration; Materials Science; Natural Materials; Oxygen - metabolism; Plates; Polymer Sciences; Prostheses and Implants; Regenerative Medicine/Tissue Engineering; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - physiology; Staphylococcus epidermidis; Staphylococcus epidermidis - drug effects; Staphylococcus epidermidis - physiology; Surfaces and Interfaces; Surgical implants; Thin Films; Bacterial Adhesion; Bioactive Glass; PTFE; Hypoxic Condition; PDMS
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-015-5569-1

To study the effect of bioactive glass bone substitute granules (S53P4) on bacterial adhesion and biofilm formation on other simultaneously used implant materials and the role of the hypoxic conditions to the adhesion. Bacterial and biofilm formation were studied on materials used both in middle ear prostheses and in fracture fixtures (titanium, polytetrafluoroethylene, polydimethylsiloxane and bioactive glass plates) in the presence or absence of S53P4 granules. The experiments were done either in normal atmosphere or in hypoxia simulating atmospheric conditions of middle ear, mastoid cavity and sinuses. We used two collection strains of Staphylococcus aureus and Staphylococcus epidermidis . In the presence of bioglass and hypoxic conditions the adhesion of the planktonic bacterial cells was decreased for most of the materials. The biofilm formation was decreased for S. epidermidis on titanium and polydimethylsiloxane in both atmospheric conditions and on bioglass plates in normoxia. For S. aureus the biofilm formation was decreased on bioglass plates and polytetrafluoroethylene in normoxia. Hypoxia produces a decrease in the biofilm formation only for S. aureus on polytetrafluoroethylene and for S. epidermidis on bioglass plates. However, in none of the cases bioactive glass increased the bacterial or biofilm adhesion. The presence of bioglass in normoxic and hypoxic conditions prevents the bacterial and biofilm adhesion on surfaces of several typical prosthesis materials in vitro. This may lead to diminishing postoperative infections, however, further in vivo studies are needed.