RPL3L-containing ribosomes determine translation elongation dynamics required for cardiac function

Although several ribosomal protein paralogs are expressed in a tissue-specific manner, how these proteins affect translation and why they are required only in certain tissues have remained unclear. Here we show that RPL3L, a paralog of RPL3 specifically expressed in heart and skeletal muscle, influe...

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Published inNature communications Vol. 14; no. 1; p. 2131
Main Authors Shiraishi, Chisa, Matsumoto, Akinobu, Ichihara, Kazuya, Yamamoto, Taishi, Yokoyama, Takeshi, Mizoo, Taisuke, Hatano, Atsushi, Matsumoto, Masaki, Tanaka, Yoshikazu, Matsuura-Suzuki, Eriko, Iwasaki, Shintaro, Matsushima, Shouji, Tsutsui, Hiroyuki, Nakayama, Keiichi I.
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 20.04.2023
Nature Publishing Group
Nature Portfolio
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Summary:Although several ribosomal protein paralogs are expressed in a tissue-specific manner, how these proteins affect translation and why they are required only in certain tissues have remained unclear. Here we show that RPL3L, a paralog of RPL3 specifically expressed in heart and skeletal muscle, influences translation elongation dynamics. Deficiency of RPL3L-containing ribosomes in RPL3L knockout male mice resulted in impaired cardiac contractility. Ribosome occupancy at mRNA codons was found to be altered in the RPL3L-deficient heart, and the changes were negatively correlated with those observed in myoblasts overexpressing RPL3L. RPL3L-containing ribosomes were less prone to collisions compared with RPL3-containing canonical ribosomes. Although the loss of RPL3L-containing ribosomes altered translation elongation dynamics for the entire transcriptome, its effects were most pronounced for transcripts related to cardiac muscle contraction and dilated cardiomyopathy, with the abundance of the encoded proteins being correspondingly decreased. Our results provide further insight into the mechanisms and physiological relevance of tissue-specific translational regulation. RPL3L is a paralog of the ribosomal protein RPL3 and specifically expressed in heart and skeletal muscle. Here, the authors show that RPL3L-containing ribosomes regulate translation elongation dynamics especially for the transcripts related to cardiac muscle contraction.
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ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-023-37838-6