A metagenomic insight into the microbiomes of geothermal springs in the Subantarctic Kerguelen Islands

• Published in: Scientific reports Vol. 12; no. 1; pp. 22243 - 12
• Main Authors: Allioux, Maxime, Yvenou, Stéven, Merkel, Alexander, Cozannet, Marc, Aubé, Johanne, Pommellec, Jolann, Le Romancer, Marc, Lavastre, Véronique, Guillaume, Damien, Alain, Karine
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 23.12.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/114; 631/158; 631/208; 631/326; Archaea; Biodiversity and Ecology; Environmental Sciences; Heterotrophs; Hot springs; Hot Springs - microbiology; Humanities and Social Sciences; Hyperthermophiles; Islands; Metagenome; Metagenomics; Metagenomics - methods; Microbiomes; Microbiota - genetics; Microorganisms; multidisciplinary; New genera; Next-generation sequencing; Phylogeny; Science; Science (multidisciplinary); Sulfur; Sulfur compounds; Sulfur cycle
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-26299-4

The Kerguelen Islands, located in the southern part of the Indian Ocean, are very isolated geographically. The microbial diversity and communities present on the island, especially associated to geothermal springs, have never been analyzed with high-throughput sequencing methods. In this article, we performed the first metagenomics analysis of microorganisms present in Kerguelen hot springs. From four hot springs, we assembled metagenomes and recovered 42 metagenome-assembled genomes, mostly associated with new putative taxa based on phylogenomic analyses and overall genome relatedness indices. The 42 MAGs were studied in detail and showed putative affiliations to 13 new genomic species and 6 new genera of Bacteria or Archaea according to GTDB. Functional potential of MAGs suggests the presence of thermophiles and hyperthermophiles, as well as heterotrophs and primary producers possibly involved in the sulfur cycle, notably in the oxidation of sulfur compounds. This paper focused on only four of the dozens of hot springs in the Kerguelen Islands and should be considered as a preliminary study of the microorganisms inhabiting the hot springs of these isolated islands. These results show that more efforts should be made towards characterization of Kerguelen Islands ecosystems, as they represent a reservoir of unknown microbial lineages.