Apelin-13 Attenuates Traumatic Brain Injury-Induced Damage by Suppressing Autophagy

• Published in: Neurochemical research Vol. 40; no. 1; pp. 89 - 97
• Main Authors: Bao, Hai-Jun, Zhang, Lin, Han, Wen-Can, Dai, Ding-Kun
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.01.2015; Springer Nature B.V
• Subjects: Animals; Autophagy - drug effects; Behavior, Animal; Biochemistry; Biomedical and Life Sciences; Biomedicine; Brain Injuries - drug therapy; Brain Injuries - pathology; Brain Injuries - psychology; Cell Biology; Cell Count; Cerebral Cortex - pathology; Hippocampus - pathology; Intercellular Signaling Peptides and Proteins - therapeutic use; Male; Maze Learning; Mice; Movement Disorders - physiopathology; Movement Disorders - psychology; Neurochemistry; Neurology; Neurosciences; Original Paper; Autophagy; Apelin-13; Traumatic brain injury (TBI)
• ISSN: 0364-3190; 1573-6903
• DOI: 10.1007/s11064-014-1469-x

The adipocytokine apelin is a peptide, Apelin and its receptor are abundantly expressed in the nervous and cardiovascular systems. Previous studies had found apelin-13 reduces brain injuries and postischemic cerebral edema through blocking programmed cell death, Apelin-13 is also able to inhibit glucose deprivation induced cardiomyocyte autophagy in a concentration dependent fashion. To observe the effect of Apelin-13 on the brain injury induced by traumatic brain injury (TBI), and explore the effect of Apelin-13 on autophagy in TBI, We performed The neurological test, and the numbers of TBI-induced neural cell death were also counted by propidium iodide labeling. At last, the autophagy associated proteins LC3, Beclin-1, Bcl-2, p62 were also assessed with western-blotting. Compared with saline vehicle groups, the neural cell death, lesion volume, and neural dysfunction were attenuated by apelin-13 after TBI. In additionally, Apelin-13 also reversed TBI induced downregulation of LC3, Beclin-1, Bcl-2, p62 expression, compared with saline vehicle groups, at 24 and 48 h post TBI. Apelin-13 attenuates TBI induced brain damage by suppressing autophagy. All these results revealed that Apelin-13 suppressed autophagy. The autophagy may be involved in the mechanism of Apelin-13 rescue the subsequent damaged neuron in TBI.