O-GlcNAcylation regulates neurofilament-light assembly and function and is perturbed by Charcot-Marie-Tooth disease mutations

• Published in: Nature communications Vol. 14; no. 1; pp. 6558 - 17
• Main Authors: Huynh, Duc T., Tsolova, Kalina N., Watson, Abigail J., Khal, Sai Kwan, Green, Jordan R., Li, Di, Hu, Jimin, Soderblom, Erik J., Chi, Jen-Tsan, Evans, Chantell S., Boyce, Michael
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 17.10.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 13/106; 13/44; 13/95; 14/1; 14/19; 38/109; 631/378/87; 631/45/221; 631/80/128/1580; 631/80/221; 631/80/86/2369; 82/58; 96/35; Acetylglucosamine; Assembly; Charcot-Marie-Tooth disease; Charcot-Marie-Tooth Disease - genetics; Charcot-Marie-Tooth Disease - pathology; Cytoskeleton; Glycosylation; Humanities and Social Sciences; Humans; Intermediate Filaments; Intracellular; multidisciplinary; Mutation; N-Acetylglucosamine; Neurodegenerative diseases; Neurofilaments; O-GlcNAcylation; Protein interaction; Protein Processing, Post-Translational; Proteins; Science; Science (multidisciplinary)
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-023-42227-0

The neurofilament (NF) cytoskeleton is critical for neuronal morphology and function. In particular, the neurofilament-light (NF-L) subunit is required for NF assembly in vivo and is mutated in subtypes of Charcot-Marie-Tooth (CMT) disease. NFs are highly dynamic, and the regulation of NF assembly state is incompletely understood. Here, we demonstrate that human NF-L is modified in a nutrient-sensitive manner by O-linked-β- N -acetylglucosamine (O-GlcNAc), a ubiquitous form of intracellular glycosylation. We identify five NF-L O-GlcNAc sites and show that they regulate NF assembly state. NF-L engages in O-GlcNAc-mediated protein-protein interactions with itself and with the NF component α-internexin, implying that O-GlcNAc may be a general regulator of NF architecture. We further show that NF-L O-GlcNAcylation is required for normal organelle trafficking in primary neurons. Finally, several CMT-causative NF-L mutants exhibit perturbed O-GlcNAc levels and resist the effects of O-GlcNAcylation on NF assembly state, suggesting a potential link between dysregulated O-GlcNAcylation and pathological NF aggregation. Our results demonstrate that site-specific glycosylation regulates NF-L assembly and function, and aberrant NF O-GlcNAcylation may contribute to CMT and other neurodegenerative disorders. The neurofilament cytoskeleton is critical for axon function and can be dysregulated in disease. Here, the authors show that intracellular glycosylation regulates a key neurofilament protein.