Shinorine and porphyra-334 isolated from laver (Porphyra dentata) inhibit adipogenesis in 3T3-L1 cells

• Published in: Food science and biotechnology Vol. 31; no. 5; pp. 617 - 625
• Main Authors: Choi, Su-Young, Lee, Su Yeon, Kim, Hyung Gyun, Jeong, Jae Cheon, Batara, Don Carlo, Kim, Sung-Hak, Cho, Jeong-Yong
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01.05.2022; Springer Nature B.V; 한국식품과학회
• Subjects: Adipocytes; Adipogenesis; Adiponectin; Amino acids; Antioxidants; Bioactive compounds; biotechnology; CCAAT/enhancer-binding protein; Chemistry; Chemistry and Materials Science; Cytotoxicity; dose response; edible seaweed; Food Science; Gene expression; Leptin; Lipids; Magnetic resonance spectroscopy; Mycosporine-like amino acids; NMR; NMR spectroscopy; Nuclear magnetic resonance; nuclear magnetic resonance spectroscopy; Nutrition; Porphyra; Preadipocytes; radiation resistance; Research Article; Shinorine; 식품과학; Laver; Adipocyte differentiation; Shinorine; Porphyra-334; Adipogenesis; Porphyra dentata
• ISSN: 1226-7708; 2092-6456; 2092-6456
• DOI: 10.1007/s10068-022-01055-6

Mycosporine-like amino acids (MAAs) such as shinorine and porphyra-334 from Porphyra spp. are bioactive compounds with strong photoprotective and antioxidant properties. In this study, the anti-adipogenic effect of shinorine and porphyra-334 was examined in vitro utilizing 3T3-L1 preadipocytes. Shinorine and porphyra-334 were extracted from laver ( Porphyra dentata ) 50% methanolic (MeOH) extract of and their structures were elucidated by MS and NMR spectroscopy. Both compounds had no cytotoxic effect in 3T3-L1 cells (< 200 μg/mL) and inhibited the accumulation of lipid droplets in 3T3-L1 mature adipocytes in a dose-dependent manner (0.1 and 1.0 μM). Interestingly, both compounds had also significantly reduced the expression of adipogenic-related genes such as peroxisome proliferator-activated receptor γ2 (PPARγ2), CCAAT/enhancer-binding protein α (C/EBPα), adiponectin, and leptin in 3T3-L1 cells. The findings suggest that shinorine and porphyra-334 have the potential to inhibit adipogenesis in 3T3-L1 preadipocytes.