Novel enzymes for biodegradation of polycyclic aromatic hydrocarbons identified by metagenomics and functional analysis in short-term soil microcosm experiments

• Published in: Scientific reports Vol. 14; no. 1; p. 11608
• Main Authors: Nagy, Kinga K., Takács, Kristóf, Németh, Imre, Varga, Bálint, Grolmusz, Vince, Molnár, Mónika, Vértessy, Beáta G.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21.05.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/114; 631/45; 631/61; 704/158; Animal husbandry; Anthracene; Biodegradation; Biodegradation, Environmental; Bioinformatics; Biotechnology; Catalysis; Dioxygenases - chemistry; Dioxygenases - genetics; Dioxygenases - metabolism; Enzymes; Experimental design; Humanities and Social Sciences; Metagenome; Metagenomic data search; Metagenomics; Metagenomics - methods; Microcosms; Microflora; Microorganisms; multidisciplinary; Naphthalene; Naphthalenes - metabolism; Novel enzymes; Oxidants; Oxidizing agents; Phenanthrene; Phenanthrenes - metabolism; Pollution control; Polycyclic aromatic hydrocarbons; Polycyclic Aromatic Hydrocarbons - metabolism; Science; Science (multidisciplinary); Soil; Soil - chemistry; Soil contamination; Soil degradation; Soil Microbiology; Soil microorganisms; Soil Pollutants - metabolism; Soil pollution; Soil remediation; Structure-function relationships; Biodegradation; Soil; Metagenomic data search; Novel enzymes; Polycyclic aromatic hydrocarbons; Bioinformatics
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-61566-6

Polycyclic aromatic hydrocarbons (PAHs) are highly toxic, carcinogenic substances. On soils contaminated with PAHs, crop cultivation, animal husbandry and even the survival of microflora in the soil are greatly perturbed, depending on the degree of contamination. Most microorganisms cannot tolerate PAH-contaminated soils, however, some microbial strains can adapt to these harsh conditions and survive on contaminated soils. Analysis of the metagenomes of contaminated environmental samples may lead to discovery of PAH-degrading enzymes suitable for green biotechnology methodologies ranging from biocatalysis to pollution control. In the present study, our goal was to apply a metagenomic data search to identify efficient novel enzymes in remediation of PAH-contaminated soils. The metagenomic hits were further analyzed using a set of bioinformatics tools to select protein sequences predicted to encode well-folded soluble enzymes. Three novel enzymes (two dioxygenases and one peroxidase) were cloned and used in soil remediation microcosms experiments. The experimental design of the present study aimed at evaluating the effectiveness of the novel enzymes on short-term PAH degradation in the soil microcosmos model. The novel enzymes were found to be efficient for degradation of naphthalene and phenanthrene. Adding the inorganic oxidant CaO 2 further increased the degrading potential of the novel enzymes for anthracene and pyrene. We conclude that metagenome mining paired with bioinformatic predictions, structural modelling and functional assays constitutes a powerful approach towards novel enzymes for soil remediation.