Erythritol alters gene transcriptome signatures, cell growth, and biofilm formation in Staphylococcus pseudintermedius

• Published in: BMC veterinary research Vol. 19; no. 1; pp. 1 - 146
• Main Authors: Fujii, Tadashi, Tochio, Takumi, Nishifuji, Koji
• Format: Journal Article
• Language: English
• Published: London BioMed Central Ltd 07.09.2023; BioMed Central; BMC
• Subjects: Analysis; Biofilm; Biofilms; Carbohydrates; Control; Dosage and administration; Erythritol; Gene expression; Gene regulation; Genes; Genetic aspects; Genetic research; Genetic transcription; Glucose; Glucose phosphotransferase; Growth; Growth inhibition; Identification and classification; Immunosuppression; Metabolism; Methicillin; Microbial mats; Microorganisms; Patient outcomes; Phosphates; Phosphotransferase; Proteins; Reverse transcription; RNA; RNA sequencing; Staphylococcus; Staphylococcus pseudintermedius; Transcriptome; Transcriptomes; Up-regulation; Japan
• ISSN: 1746-6148; 1746-6148
• DOI: 10.1186/s12917-023-03711-3

Abstract Background Erythritol was found to inhibit the growth of microorganisms. The present study aimed to demonstrate the growth inhibition of Staphylococcus pseudintermedius by erythritol and to define the changes in gene transcription signatures induced by erythritol. Changes in the gene transcription profiles were analysed by RNA sequencing and quantitative reverse transcription PCR. Gene ontology analysis was performed to assign functional descriptions to the genes. Results Erythritol inhibited S. pseudintermedius growth in a dose-dependent manner. We then performed a transcriptome analysis of S. pseudintermedius with and without 5% (w/w) erythritol exposure to validate the mechanism of growth inhibition. We revealed that erythritol induced up-regulation of three genes ( ptsG , ppdK , and ppdkR ) that are related to the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). Glucose supplementation restored the up-regulation of the PTS-related genes in response to erythritol. In addition, erythritol down-regulated eleven genes that are located in a single pur -operon and inhibited biofilm formation of S. pseudintermedius . Conclusions These findings indicated that erythritol antagonistically inhibits PTS-mediated glucose uptake, thereby exerting a growth inhibitory effect on S. pseudintermedius . Moreover, erythritol inhibits the ‘de novo’ IMP biosynthetic pathway that may contribute to biofilm synthesis in S. pseudintermedius .