Histological mapping of biochemical changes in solid tumors by FT-IR spectral imaging

• Published in: FEBS letters Vol. 581; no. 28; pp. 5469 - 5474
• Main Authors: Petibois, Cyril, Drogat, Benjamin, Bikfalvi, Andreas, Déléris, Gérard, Moenner, Michel
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 27.11.2007
• Subjects: CAM; CAM-assay; Cancer; Cell Hypoxia - drug effects; Cell Line, Tumor; chorio-allantoic membrane; Fourier-transform infrared; FT-IR; FT-IR imaging; Glucose - pharmacology; Histopathology; Humans; Metabolism; Neoplasm Transplantation; Neoplasms - blood supply; Neoplasms - metabolism; Neoplasms - pathology; Oxidative stress; Spectroscopy, Fourier Transform Infrared - methods; Oxidative stress; Histopathology; CAM-assay; FT-IR; FT-IR imaging; Metabolism; CAM; Cancer
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2007.10.052

Fourier-transform infrared (FT-IR) spectral imaging was used for analyzing biochemical changes in tumor cells. Metabolic parameters of human lung A549/8 adenocarcinoma and U87 glioma cells were compared under stress conditions in culture along with tumor progression after cell implantation onto the chick embryo chorio-allantoic membrane. In cell culture, glucose consumption and lactic acid release were higher in U87 cells. A549/8 cells were less sensitive to oxidative stress as observed through changes in fatty acyl chains. In vivo biochemical mapping of highly (U87) vs. poorly (A549/8) angiogenic tumors provided results comparable to culture models. Therefore, FT-IR imaging allows detecting subtle chemical changes in tumors, which might be useful for diagnosis.