Tumor-infiltrating lymphocytes derived from human renal cell carcinoma: Clonal analysis of its characteristics

• Published in: International journal of urology Vol. 15; no. 3; pp. 241 - 244
• Main Authors: Shimabukuro, Tomoyuki, Naito, Katsusuke
• Format: Journal Article
• Language: English
• Published: Melbourne, Australia Blackwell Publishing Asia 01.03.2008
• Subjects: Carcinoma, Renal Cell; CD4 antigen; Cells, Cultured; clone; Clone Cells; Cytological Techniques; human renal cell carcinoma; Humans; Kidney Neoplasms; Lymphocytes, Tumor-Infiltrating; tumor-infiltrating lymphocytes
• ISSN: 0919-8172; 1442-2042
• DOI: 10.1111/j.1442-2042.2007.01977.x

Aim:  To assess the characteristics of activated tumor‐infiltrating lymphocytes (TIL), we report the isolation, growth response, and functional analysis of a CD4‐ CD8+ TIL‐clone derived from human renal cell carcinoma (RCC). Methods:  Bulk TILs were expanded from a human RCC and the lymphocytes were separated into a CD8+ enriched population. Subsequently, using the limiting dilution technique, a TIL clone was established and its growth response, phenotype and cytotoxic activity were analyzed. Results:  A clone, T16‐13, by day 94 numbering 1 × 107 cells, was harvested and characterized as a CD4‐ CD8+ clone. On day 144, the cytotoxic activity of this clone against the autologous tumor was relatively high (2.3 ± 0.7 LU30/106 cells). Meanwhile, against allogeneic renal tumors, there was no cytotoxic activity (−0.1 LU30/106 cells). Conclusions:  A TIL clone possessing modest autologous tumor‐specific cytotoxicity can be isolated from human RCC. The characteristics analysis of various TIL clones may provide a better understanding of an RCC tumor microenvironment and may help to establish new modalities for the treatment of patients with metastatic kidney cancer.