Evaluation of a combined linear-nonlinear approach for column characterization using modern alkaline-stable columns as model

• Published in: Journal of separation science Vol. 36; no. 11; pp. 1753 - 1761
• Main Authors: Undin, Torgny, Samuelsson, Jörgen, Törncrona, Anders, Fornstedt, Torgny
• Format: Journal Article
• Language: English
• Published: Weinheim Blackwell Publishing Ltd 01.06.2013; Wiley; Wiley Subscription Services, Inc
• Subjects: Adsorption; Adsorption isotherms; Analytical chemistry; Characterization; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography; Exact sciences and technology; Hybrid systems; Isotherms; Kemi; Linear methods; Mathematical analysis; Mathematical models; Nonlinear methods; Nonlinearity; Other chromatographic methods; Retention; Characterization; Reversed phase chromatography; Chromatographic retention; Adsorption isotherm; HPLC chromatography; Adsorption isotherms; Nonlinear methods; Linear methods; Retention; Bonded stationary phase
• ISSN: 1615-9306; 1615-9314; 1615-9314
• DOI: 10.1002/jssc.201201132

This study investigates if deeper understanding is achieved when combining nonlinear and linear chromatographic column characterization methods. As test systems, two hybrid columns (Phenomenex Gemini‐NX C18 and Kromasil Eternity C18) and one classic one (Kromasil‐C18) were selected. The nonlinear methods were based on firm adsorption theory and involved determination of adsorption isotherms followed by calculations with a new numerical tool, adsorption energy distribution, on probe components at different pH values. The linear methods involved the hydrophobic subtraction model and selected probe components retention factors as a function of pH. The combined analysis indicated that both complementary and confirmative information can be achieved regarding the actual model systems.