Endogenous polyamine content and metabolism in the ectomycorrhizal fungus Paxillus involutus

• Published in: The New phytologist Vol. 143; no. 3; pp. 581 - 587
• Main Authors: FORNALÉ, SILVIA, SARJALA, TYTTI, BAGNI, NELLO
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.09.1999; Blackwell
• Subjects: age; arginine decarboxylase; Biological and medical sciences; Biosynthesis; cadaverine; carboxy-lyases; chemical composition; enzyme activity; enzyme inhibitors; Enzymes; Forest soils; Fundamental and applied biological sciences. Psychology; Fungi; Growth, nutrition, metabolism, transports, enzymes. Molecular biology; Liquids; Metabolism; Microbiology; Mycelium; Mycology; ornithine decarboxylase; Paxillus involutus; Plants; polyamine release; polyamine uptake; Polyamines; putrescine; quantitative analysis; spermidine; Symbiosis; uptake; Paxillus involutus; ornithine decarboxylase; arginine decarboxylase; polyamine uptake; polyamines; polyamine release; Symbiosis; Cadaverine; Polyamine; Symbionte; Arginine decarboxylase; Enzyme; Basidiomycetes; Lyases; Biosynthesis; Ornithine decarboxylase; Concentration; Putrescine; Metabolism; Fungi; Carbon-carbon lyases; Absorption; Enzymatic activity; Carboxy-lyases; Spermidine; Mycorrhiza; Ectomycorrhiza; Release; Thallophyta
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1046/j.1469-8137.1999.00480.x

Endogenous polyamine content of the ectomycorrhizal fungus Paxillus involutus, as well as the activity of its biosynthetic enzymes in relation to mycelia ageing were investigated in this work. Polyamines in free, PCA-soluble and insoluble conjugated forms, are present in Paxillus involutus mycelia in relatively high amounts and the ratio of putrescine to spermidine is age-dependent. Both arginine- and ornithine-decarboxylases are present, but putrescine biosynthesis proceeds mostly via ornithine decarboxylase and decreases with the age of mycelia. There was a large release of free polyamines from mycelia which showed age-dependent features. Clear polyamine uptake was observed in 2-wk-old mycelia and no competition between putrescine and cadaverine was detected. Putrescine uptake seems to reduce ornithine decarboxylase activity, but does not affect arginine decarboxylase.