CTP—OD1—HA和CTP—OD2-HA融合肽在K562细胞中的定位及其与BCR—ABL蛋白的相互作用

目的研究CTP—OD1-HAS和CTP．OD2．HA两种融合肽在慢性粒细胞白血病（CML）K562细胞中的定位及其与BCR．ABL蛋白的相互作用。方法将前期制备的CTP—OD1-HA和CTP-OD2．HA融合肽转导／KK562细胞，采用免疫荧光和Westernblotting方法检测其人胞情况及胞内定位，His．pulldown和CoIP实验检测融合肽与BCR．ABL的相互作用。结果免疫荧光检测显示CTP—ODI—HA和CTP—OD2-HAlt合肽均能穿过细胞膜进入细胞并定位于胞质，Western blotting也同时检测到进入细胞质中的两种融合肽。His-pull down实验结果表明．C...

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Published inJie fang jun yi xue za zhi Vol. 38; no. 11; pp. 896 - 899
Main Author 肖恒 王海霞 陶崑 刘鑫 钟梁 黄世峰 冯文莉
Format Journal Article
LanguageChinese
Published Beijing People's Military Medical Press 2013
Subjects
Cytoplasm
Peptides
Proteins
白血病，粒，单核细胞，慢性
融合蛋白质类，bcr-abl
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ISSN0577-7402
DOI10.11855/j.issn.0577-7402.2013.11.006

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Summary:目的研究CTP—OD1-HAS和CTP．OD2．HA两种融合肽在慢性粒细胞白血病（CML）K562细胞中的定位及其与BCR．ABL蛋白的相互作用。方法将前期制备的CTP—OD1-HA和CTP-OD2．HA融合肽转导／KK562细胞，采用免疫荧光和Westernblotting方法检测其人胞情况及胞内定位，His．pulldown和CoIP实验检测融合肽与BCR．ABL的相互作用。结果免疫荧光检测显示CTP—ODI—HA和CTP—OD2-HAlt合肽均能穿过细胞膜进入细胞并定位于胞质，Western blotting也同时检测到进入细胞质中的两种融合肽。His-pull down实验结果表明．CTP—OD1-HA和CTP—OD2-HA融合肽在细胞外均可直接与BCR-ABL蛋白结合，ColP实验结果显示两种融合肽在K562细胞内也能与BCR-ABL蛋白结合并形成复合物。结论CTP-OD1-HA和CTP—OD2-HA融合肽均能成功进入白血病KS62细胞并定位于细胞质，且可与BCR．ABL蛋白发生相互作用。
Bibliography:leukemia, myelomonocytic, chronic; fusion proteins, bcr-abl
XIAO Heng, WANG Hai-xia, TAO Kun, LIU Xin, ZHONG Liang, HUANG Shi-feng, FENG Wen-li（ Department of Clinical Hematology, Key Laboratory of Laboratory Medical of Education Ministry, Chongqing Medical University, Chongqing 400016, China）
11-1056/R
Objective To investigate the intracellular location of cytoplasmic transduction peptide-oligomerization domain 1-hemagglutinin （CTP-OD1-HA） and cytoplasmic transduction peptide-oligomerization domain 2-hemagglutinin （CTP-OD2-HA） fusion peptide and interaction with BCR-ABL protein in chronic myelocytic leukemia （CML） K562 cells. Methods The prepared CTP-OD1-HA and CTP-OD2-HA fusion peptide were transduced into K562 cells, and the cytoplasmic transduction peptide- oligomerization domain-hemagglutinin （CTP-OD-HA） peptide was used as positive control, and cytoplasmic transduction peptide- hemagglutinin （CTP-HA） and PBS were used as negative control. The intracellular location of the fusion peptide was demonstrated by
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ISSN:0577-7402
DOI:10.11855/j.issn.0577-7402.2013.11.006